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Ruti Sella, Orly Gal-Or, Assaf Dotan, Mor Dachbash, Yael Nisgav, Dov Weinberger, Rita Ehrlich, Tami Livnat; Clearance of Aflibercept Following Intravitreal Injection in a Rat Model. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3326.
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© ARVO (1962-2015); The Authors (2016-present)
Aflibercept is known as a potent anti vascular endothelial growth factor (anti-VEGF), though little is known regarding its clearance mechanism following intravitreal injection to the eye. Our purpose was to characterize the localization and clearance of intravitreally injected Aflibercept in the eye in a rat model.
Choroidal neovascularization (CNV) was induced by diode laser photocoagulation on the right eye of 8 Brown Norway rats.3µl Aflibercept (25mg/ml) was injected intravitreally on day 3 from CNV induction. Immediately after Aflibercept injection and 3,6,24 and 48 hours later, animals were euthanized and eyes were enucleated for immunofluorescence staining. Donkey anti-human IgG labeled with Alexa Fluor® 488 was used for Aflibercept immunoreactivity detection. Untreated eyes were used as negative control. Anti CD31 antibody was used as a marker for schlemm's canal's endothelial cells. Intensity of the immunofluorescence staining was analyzed qualitatively.
Aflibercept immunoreactivity was detected in the cornea, iridocorneal angle, and the retina immediately after injection, and declined in a decremented manner within the following hours.Forty eight hours from the injection no Aflibercept staining was detected in the structures mentioned above.
Our study demonstrated the immediate accumulation of intravitreally injected Aflibercept in retina, cornea and iridocorneal angle and its presence there at least for 24 hours.Clearance of Aflibercept molecules from the eye through the iridocorneal angle and retina were shown to take place within 48 hours.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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