September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Time course of oxidative stress markers and inflammatory mediators in human conjunctival epithelial cells exposed to diesel exhaust particles
Author Affiliations & Notes
  • Romina M. Lasagni Vitar
    General and Inorganic Chemistry Division, IBIMOL (UBA-CONICET), Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Julia Tau
    Ocular Investigation Laboratory, Pathology Department, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
  • Agustina Tesone
    Ocular Investigation Laboratory, Pathology Department, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
  • Natasha Janezic
    General and Inorganic Chemistry Division, IBIMOL (UBA-CONICET), Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Ailen Hvozda Arana
    General and Inorganic Chemistry Division, IBIMOL (UBA-CONICET), Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Claudia Reides
    General and Inorganic Chemistry Division, IBIMOL (UBA-CONICET), Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Alejandro Berra
    Ocular Investigation Laboratory, Pathology Department, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
  • Sandra M Ferreira
    General and Inorganic Chemistry Division, IBIMOL (UBA-CONICET), Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Susana Llesuy
    General and Inorganic Chemistry Division, IBIMOL (UBA-CONICET), Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships   Romina Lasagni Vitar, None; Julia Tau, None; Agustina Tesone, None; Natasha Janezic, None; Ailen Hvozda Arana, None; Claudia Reides, None; Alejandro Berra, None; Sandra Ferreira, None; Susana Llesuy, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3508. doi:
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    • Get Citation

      Romina M. Lasagni Vitar, Julia Tau, Agustina Tesone, Natasha Janezic, Ailen Hvozda Arana, Claudia Reides, Alejandro Berra, Sandra M Ferreira, Susana Llesuy; Time course of oxidative stress markers and inflammatory mediators in human conjunctival epithelial cells exposed to diesel exhaust particles. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3508.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Ocular discomfort due to urban air pollution has been well documented. Diesel Exhaust Particles (DEP) are the main component of urban particulate matter. The aims of the present study were to evaluate oxidative stress markers and inflammatory mediators in human conjunctival epithelial cells exposed to DEP for 1 and 3 h, and to compare these results with the previously reported for a 24 h incubation

Methods : Human conjunctival epithelial cells (IOBA-NHC) were incubated at different concentrations of DEP (10, 50 and 100 μg/mL.Control: culture medium) for 1, 3 and 24 h. The following parameters were evaluated: reactive oxygen (ROS) and nitrogen species (RNS) production, protein and lipid oxidation, superoxide dismutase (SOD) activity, reduced glutathione (GSH) and IL-6 and IL-8 levels. One-way ANOVA test and Tukey’s test as post hoc test were used for statistical analysis

Results : Compared to the control group, the cells exposed to 50 and 100 μg/mL of DEP displayed an increase in ROS (1 h: 64% and 91% 3 h: 31% and 46% 24 h: 22% and 45%, p<0.01) and RNS (1 h: 229% and 500% 3 h: 214% and 457% 24 h: 257% and 514%, p<0.01). SOD activity significantly increased at all time points evaluated (1 h: 36% and 42% 3 h: 84% and 90% 24 h: 85% and 110%, p<0.05). The same experimental groups showed an increase in GSH levels after 3 h of incubation (30% and 41%, p<0.05), but GSH diminished after 24 h (39% and 50% p<0.05). DEP50 and DEP100 groups also presented an increase in lipid oxidation after 1 h of incubation (66% and 79%, p<0.05). Protein oxidation was increased in DEP100 group after 3 and 24 h of incubation (48% and 57%, p<0.05). Higher values of IL-6 release were observed in DEP100 group after the third hour of incubation (54%, p<0.05). Meanwhile, IL-8 levels were significantly decreased after the first hour of incubation (46%, p<0.01). Both cytokines showed the same pattern after 24 h of incubation. There was no significant difference between DEP10 and control groups at all time points evaluated

Conclusions : These results suggest that ROS and RNS production seem to be earlier events triggered by the exposure to DEP in human conjunctival epithelial cells, in comparison to the proinflammatory response mediated by IL-6. In these conditions, the antioxidant mechanisms activated were not enough to avoid oxidative damage to macromolecules, developing in cell toxicity

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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