September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Anti-inflammatory and anti-fibrotic effects of novel peptide GQDGLAGPK in alkaline burns rabbit
Author Affiliations & Notes
  • Lee Yoon Jin
    Inje university Busan paik hospital, Busan, Korea (the Republic of)
  • Hye Sook Lee
    Inje university Busan paik hospital, Busan, Korea (the Republic of)
  • YuJin Lee
    Inje university Busan paik hospital, Busan, Korea (the Republic of)
  • JaeWook Yang
    Inje university Busan paik hospital, Busan, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Lee Yoon Jin, None; Hye Sook Lee, None; YuJin Lee, None; JaeWook Yang, None
  • Footnotes
    Support  This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health and Welfare Affairs, Republic of Korea (grant #: HI12C0005)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3515. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Lee Yoon Jin, Hye Sook Lee, YuJin Lee, JaeWook Yang; Anti-inflammatory and anti-fibrotic effects of novel peptide GQDGLAGPK in alkaline burns rabbit. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3515.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the effect of novel peptide GQDGLAGPK from extracellular matrix (ECM) composite produced by chondrocytes in alkaline burns rabbit.

Methods : Corneal neovascularization (NV) was induced by 1 N NaOH to the right central corneas of rabbits. Seven days later, GQDGLAGPK were treatment onto the corneal surface to completely eyedrop. On the 10th day after treatment, we investigated the fibrosis, NV, inflammation and structural changes of the cornea. We examined the effects of GQDGLAGPK on clinical NV features and on the expression of corneal NV markers. In addition, we performed western blotting for expression of nuclear transcription factors, MAPKs, Akt, PKC and signaling molecules.

Results : The alkaline burn produced significant NV and increased corneal thickness. On day 10 after treatment, corneal neovascularization (1.4±0.5) and thickness (876.0±37.0 μm) of the cornea were markedly decreased (p<0.05). However, GQDGLAGPK treatment suppressed the inflammation, fibrosis and NV in the stroma following by alkaline burn. Furthermore, the GQDGLAGPK treatment decreased the expression of angiogenic and inflammatory hallmarks. The GQDGLAGPK suppressed corneal NV by inhibiting nuclear factor-kappa B (NF-κB) activation through blocking the MAPKs, Akt, PKC signaling pathway.

Conclusions : We found that collagen type II α1 based peptide sequence GQDGLAGPK was major component from chondrocytes-derived ECM, and it was markedly effective in healing alkali-burned corneas by modulating the corneal opacity, NV, fibrosis and inflammation via blocking the NF-κB.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×