September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Induced capillary remodelling in inflammatory ocular angiogenesis model
Author Affiliations & Notes
  • Anthony Mukwaya
    Ophthalmology, Linkoping University, Linköping, Sweden
  • Beatrice Bourghardt Peebo
    Ophthalmology, Linkoping University, Linköping, Sweden
  • Maria Xeroudaki
    Ophthalmology, Linkoping University, Linköping, Sweden
  • Zaheer Ali
    Department of Medical and Health Sciences, Division of Cardiovascular Medicine, Linköping University, Linkoping, Sweden
  • Lasse Jensen
    Department of Medical and Health Sciences, Division of Cardiovascular Medicine, Linköping University, Linkoping, Sweden
    Department of Microbiology, Tumor and Cell biology, the Karolinska Institute, Stockholm, Sweden
  • Neil S Lagali
    Ophthalmology, Linkoping University, Linköping, Sweden
  • Footnotes
    Commercial Relationships   Anthony Mukwaya, Bayer (F); Beatrice Bourghardt Peebo, Bayer (F); Maria Xeroudaki, Bayer (F); Zaheer Ali, Bayer (F); Lasse Jensen, Bayer (F); Neil Lagali, Bayer (F)
  • Footnotes
    Support  Unrestricted grant from Bayer.Support from the Swedish research council (2012-2472)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3523. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Anthony Mukwaya, Beatrice Bourghardt Peebo, Maria Xeroudaki, Zaheer Ali, Lasse Jensen, Neil S Lagali; Induced capillary remodelling in inflammatory ocular angiogenesis model. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3523.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Mechanisms for microvascular remodelling and capillary maturation are not fully understood. Here, we hypothesise that in pathology, signalling pathways that lead to rapid microvascular remodelling are independent of the VEGF-A axis.

Methods : The suture-induced corneal neovascularisation model in rats was used, involving male Wistar rats sutured temporally in the cornea with two nylon sutures at 1.5mm from the limbus. When the new capillary sprouts had invaded the cornea to 50% of the distance to the suture, (time point 0h), rats were divided into a suture IN group, where sutures were maintained and corneas sampled at 24, 72 and 120h (4 cornea/time point), and a suture OUT group, in which sutures were removed at 0h to induce remodelling, and then sampled at the same time points as described above. 4 non-sutured cornea served as controls. A slit lamp camera was used to track vascular density dynamics and in vivo confocal microscopy to monitor inflammatory cell infiltration and changes in morphology of single capillaries. Microarrays were used to monitor whole transcriptome changes at 24 h time point. Analysis of variance (ANOVA) was used for group comparisons, P value < 0.05= significant.

Results : Removal of the angiogenic stimulus effected a granulocyte-to-macrophage transition (P = 0.01-to-P = 0.004), and vascular density decreased starting at 72h relative to suture IN (P = 0.04). VEGF-A expression reduced with suture removal (P<0.001). Also many other proinflammatory, proangiogenic, and promaturation genes were deferentially regulated between suture IN and suture OUT at 24h time point.

Conclusions : Early microvascular maturation in our model is not exclusively dependent upon VEGF-A, but also involves other signalling pathways of potential therapeutic interest.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×