September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Effects of Vascular Endothelial Growth Factor-A and -B on angiogenesis and nerve regeneration of the cornea
Author Affiliations & Notes
  • qiang zhou
    Ophthalmology & Visual Science, University of Illinois at Chicago, Chicago, Illinois, United States
  • Victor H Guaiquil
    Ophthalmology & Visual Science, University of Illinois at Chicago, Chicago, Illinois, United States
  • Yun Cin Luo
    Ophthalmology & Visual Science, University of Illinois at Chicago, Chicago, Illinois, United States
  • Michael Sun
    Ophthalmology & Visual Science, University of Illinois at Chicago, Chicago, Illinois, United States
  • Mark Rosenblatt
    Ophthalmology & Visual Science, University of Illinois at Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   qiang zhou, None; Victor Guaiquil, None; Yun Cin Luo, None; Michael Sun, None; Mark Rosenblatt, None
  • Footnotes
    Support  R01EY018594, RPB Career Development Award
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3531. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      qiang zhou, Victor H Guaiquil, Yun Cin Luo, Michael Sun, Mark Rosenblatt; Effects of Vascular Endothelial Growth Factor-A and -B on angiogenesis and nerve regeneration of the cornea. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3531.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To study the dose dependent effect of VEGF-induced endothelial and neuronal cell growth.

Methods : To characterize the differential outcomes by which VEGF-A and VEGF-B induced angiogenesis and cornea nerve regeneration, we study the effects of VEGF-A and -B on in vitro angiogenesis assays (cell proliferation, wound healing and tube formation) using mouse aortic endothelial cells (MAEC), as well as in vivo, using a cornea micro pellet method in which growth factor are slowly released into the cornea stroma. The blood vessel growth was traced using Image J software on Cd31 stained immunofluorescence flat mount corneas as well as in images taken in vivo. Nerve growth was traced using Neurolucida software on flat mount corneas stained with B3 tubulin.

Results : VEGF-A is a well known angiogenic factor, however at the dose used for nerve regeneration it does not induce blood vessel growth. In vitro, we found that both VEGF-A and VEGF-B induced MAEC cell proliferation, increased wound healing and tube formation independently of the dose administered. However, in vivo only VEGF-A used at 150 ng/ml induced significant blood vessel growth in the corneas treated with growth factor loaded pellets. VEGF-B used at this same dose not induce any visible angiogenesis. Similarly, both ligands used at the lower dose of 50 ng/ml which is a potent dose for nerve regeneration, does not induce neovascularization.

Conclusions : VEGF-A and -B can selectively and potently enhance neurite growth, but only VEGFA at high dose is a potent inducer of angiogenesis. VEGF-B appears as a potent and specific nerve regeneration inducer and may provide a better therapeutic to avoid unwanted angiogenesis in the injured cornea.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×