September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Oscillatory Rhythm in Degenerate Mouse (rd10) Retina Modulates Retinal Ganglion Cell Response to Electrical Stimulus
Author Affiliations & Notes
  • Dae-jin Park
    Department of Physiology, School of Medicine, Chungbuk National University, Cheongju, Korea (the Republic of)
  • JungRyul Ahn
    Department of Physiology, School of Medicine, Chungbuk National University, Cheongju, Korea (the Republic of)
  • Seong Kwang Cha
    Department of Physiology, School of Medicine, Chungbuk National University, Cheongju, Korea (the Republic of)
  • Yong Sook Goo
    Department of Physiology, School of Medicine, Chungbuk National University, Cheongju, Korea (the Republic of)
  • Solomon Senok
    Neuroscience, College of Medicine, Alfaisal University, Riyadh, Saudi Arabia
  • Footnotes
    Commercial Relationships   Dae-jin Park, None; JungRyul Ahn, None; Seong Kwang Cha, None; Yong Sook Goo, None; Solomon Senok, None
  • Footnotes
    Support  NRF-2010-0020852, NRF-2013R1A1A3009574
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3730. doi:
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      Dae-jin Park, JungRyul Ahn, Seong Kwang Cha, Yong Sook Goo, Solomon Senok; Oscillatory Rhythm in Degenerate Mouse (rd10) Retina Modulates Retinal Ganglion Cell Response to Electrical Stimulus. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3730.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Appearance of aberrant spiking activity coupled with 5 ~15 Hz oscillatory local field potential (LFP) has been reported in degenerate mouse retinas. These LFPs have been regarded as noise which reduced the efficacy of prosthetic stimulation. Since LFP varies with postnatal ages in rd10 mice, here, we investigated if the LFP might affect RGC responses to electrical stimulation in rd10 retina according to degeneration process.

Methods : We recorded electrically-evoked RGC spikes using 8×8 multi-electrode array (MEA) in rd10 mice at postnatal week (PNW) 2 ~34. Retinal patches were placed RGC layer down on MEA. Fifty cathodic phase-1st biphasic square pulses (duration: 500 µs, pulse amplitude: 5 ~60 μA) were applied at every 1 sec. Previously, 1st peak in post-stimulus time histogram (PSTH) showed better modulation efficacy than subsequently appeared remnant peaks. Thus, stimulus efficacy of PSTH 1st peak and remnant peaks was compared among different age groups to clarify potential information-carrying role of multiple peaks. Also, correlation between evoked spike numbers in PSTH peaks and LFP was calculated.

Results : Evoked spike number of PSTH 1st peak is significantly higher at PNW 6.5 (p<0.05), PNW 20 (p<0.001) and lower at PNW 10 (p<0.05) than that of all other ages except PNW 2. Modulation range of RGC response to electrical stimulus is narrower at these ages, when power of ~ 5 Hz oscillation is much higher (PNW 6.5 and 20) or lower (PNW 10) than those in other age groups. Remnant peaks show broader modulation range at PNW 8&26 than those at other ages. Power of ~5 Hz oscillation shows good correlation with evoked spike number of PSTH 1st peak in group of PNW 6.5&8 (R=0.7, p<0.001), but it has weak correlation with 1st peak in group of PNW 15 ~34 (R=0.4, p<0.05). There is no correlation between power of oscillation and evoked spike number of remnant peaks across all ages (R=0.2, p<0.05).

Conclusions : Power of ~5 Hz oscillation in rd 10 retinas modulates RGC response to electrical stimulus in PSTH 1st peak at PNW 6.5 ~8. At PNW 15 ~34, ~5 Hz oscillation has only a limited effect on PSTH 1st peak. These results suggest that power of oscillation may be an important factor determining the efficacy of electrical stimulus. Furthermore, high modulation efficacy of PSTH remnant peaks at PNW 8&26 may support the idea of information-carrying role of remnant peaks.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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