September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Human Adipose-derived Stem Cells Integrate into Normal Mouse Trabecular Meshwork
Author Affiliations & Notes
  • YI ZHOU
    Ophthalmology, University of Pittsburgh, PITTSBURGH, Pennsylvania, United States
    Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan, China
  • HONGMIN YUN
    Ophthalmology, University of Pittsburgh, PITTSBURGH, Pennsylvania, United States
  • Enzhi Yang
    Ophthalmology, University of Pittsburgh, PITTSBURGH, Pennsylvania, United States
  • Xiaobo Xia
    Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan, China
  • Joel S Schuman
    Ophthalmology, University of Pittsburgh, PITTSBURGH, Pennsylvania, United States
    Ophthalmology, McGowan Institute for Regenerative Medicine, University of Pittsburgh, PITTSBURGH, Pennsylvania, United States
  • Yiqin Du
    Ophthalmology, University of Pittsburgh, PITTSBURGH, Pennsylvania, United States
    Ophthalmology, McGowan Institute for Regenerative Medicine, University of Pittsburgh, PITTSBURGH, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   YI ZHOU, None; HONGMIN YUN, None; Enzhi Yang, None; Xiaobo Xia, None; Joel Schuman, None; Yiqin Du, None
  • Footnotes
    Support  NIH Grant R01-EY025643, P30-EY008098; BrightFocus Foundation G201486, An anonymas phalanthropic foundation, Eye&Ear Foundation of Pittsburgh, Research to Prevent Blindness
Investigative Ophthalmology & Visual Science September 2016, Vol.57, No Pagination Specified. doi:
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      YI ZHOU, HONGMIN YUN, Enzhi Yang, Xiaobo Xia, Joel S Schuman, Yiqin Du; Human Adipose-derived Stem Cells Integrate into Normal Mouse Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. 201657(12):.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have previously induced human adipose-derive stem cells (ADSCs) into trabecular meshwork cells (TMs) as a regenerative therapeutic candidate for glaucoma. This study aims to compare the effects of differentiated ADSCs and undifferentiated ADSCs after intracameral micro-injection into non-glaucomatous mouse eyes.

Methods : Human ADSCs were obtained from the Adipose Stem Center (Drs. Kacey Marra and J. Peter Rubin) at University of Pittsburgh. ADSCs were induced to differentiate into TM cells by culturing on extracellular matrix produced by TM cells in TM-conditioned media. After 14 days, both differentiated and undifferentiated ADSCs were collected and stained with membrane dye Vybrant DiO. Cells were then diluted at the density of 10,000 cells/µl in DMEM/F12, and 2µl of cells were injected at the speed of 0.22 µl/min into C57BL/6 mouse anterior chamber using a 5-µl capillary tube micropipette connected to a syringe pump. Sham injection and normal mice served as controls. Intraocular pressure (IOP) was measured at day 0, 3, 6, 10, 14, week 3, 4, 5, 6 using an iCare tonometer. Anterior chamber structure images were taken to observe inflammation outcome. Outflow facility was monitored using an ex vivo perfusion system to test the IOP-regulating function. Confocal microscopy on whole-mounts and cryosections was performed to detect the location of injected cells.

Results : At various time points post injection, there was no significant difference in IOP and outflow facility among each group. Anterior chamber structure in each group remained intact without visible vascularization or adhesion. Both differentiated and undifferentiated ADSCs homed to the TM region. The homed cells were viable and integrated into the cell layers of the original TM structure.

Conclusions : Both differentiated ADSCs with TM phenotype and undifferentiated ADSCs homed to the TM tissue and did not increase IOP after intracameral injection. Long-term effects on restoring trabecular meshwork function in glaucomatous animal models are needed for further investigation.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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