September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Long-term treatment with 9-cis-β-carotene rich alga Dunaliella Bardawil inhibits photoreceptor degeneration in a mouse model of retinoid cycle defect
Author Affiliations & Notes
  • Ifat Sher
    Goldschleger Eye Institute, Sheba Medical Center, Tel-Hashomer, Israel
    Sackler Medical School, Tel Aviv University, Tel Aviv, Israel
  • Victoria Edelshtain
    Goldschleger Eye Institute, Sheba Medical Center, Tel-Hashomer, Israel
    Sackler Medical School, Tel Aviv University, Tel Aviv, Israel
  • Adi Tzameret
    Goldschleger Eye Institute, Sheba Medical Center, Tel-Hashomer, Israel
    Sackler Medical School, Tel Aviv University, Tel Aviv, Israel
  • Dror Harats
    The Bert W. Strassburger Lipid Center, Sheba Medical Center, Tel Hashomer, Israel
  • Aviv Shaish
    The Bert W. Strassburger Lipid Center, Sheba Medical Center, Tel Hashomer, Israel
  • Ygal Rotenstreich
    Goldschleger Eye Institute, Sheba Medical Center, Tel-Hashomer, Israel
    Sackler Medical School, Tel Aviv University, Tel Aviv, Israel
  • Footnotes
    Commercial Relationships   Ifat Sher, None; Victoria Edelshtain, None; Adi Tzameret, None; Dror Harats, None; Aviv Shaish, None; Ygal Rotenstreich, None
  • Footnotes
    Support  TEVA NNE2013
Investigative Ophthalmology & Visual Science September 2016, Vol.57, No Pagination Specified. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ifat Sher, Victoria Edelshtain, Adi Tzameret, Dror Harats, Aviv Shaish, Ygal Rotenstreich; Long-term treatment with 9-cis-β-carotene rich alga Dunaliella Bardawil inhibits photoreceptor degeneration in a mouse model of retinoid cycle defect. Invest. Ophthalmol. Vis. Sci. 201657(12):.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To examine the therapeutic effect of oral treatment with 9-cis β-carotene rich alga Dunaliella Bardawil on retinal function and structure in Rpe65rd12 mice, a model of genetic defect in retinoid cycle.

Methods : Thirteen RPE65rd12 mice at age of 28 days were fed with Teklad Global 18% Protein Rodent Diet (normal diet), vitamin A deficiency diet (VAD) or VAD diet supplemented with extracts of 9-cis β-carotene rich Dunaliella Bardawil algae (DUNA) for 14 months. Retinal function was recorded once a month in both eyes simultaneously by electroretinograph (ERG). Histological analysis and immunofluorescence staining with anti- M opsin antibody were used to determine retinal structure.

Results : At 14 months, mice fed with DUNA presented significantly higher maximal a-wave coampared with mice fed with VAD or normal diet (30μV [SE=5] vs. 5 μV [SE=3] p=0.006 and vs. 3 μV [SE=2] p=0.007, respectivley) and significantly higher maximal b-wave compared with mice fed normal diet (82μV [SE=8] vs. 30 μV [SE=10] p=0.002). These results are assosicated with histological analysis revealing increased number of M-opsins in retina of mice treated with DUNA.

Conclusions : Long term oral treatment with Dunaliella powder rich in 9-cis-β-carotene preserves retinal function and rescues M-cones from degeneration in Rpe65rd12 mice. Our findings suggest that 9-cis β-carotene may possibly be an effective treatment for retinal dystrophies involving the retinoid cycle.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×