September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Identification and Characterization of Long Noncoding RNA Contributions to Mouse Corneal Development
Author Affiliations & Notes
  • Weiwei Chen
    State Key Laboratory of Ophthalmology, Wenzhou Medical University, Wenzhou, China
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Shuai Yang
    State Key Laboratory of Ophthalmology, Wenzhou Medical University, Wenzhou, China
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Zhonglou Zhou
    State Key Laboratory of Ophthalmology, Wenzhou Medical University, Wenzhou, China
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Xiaoting Zhao
    State Key Laboratory of Ophthalmology, Wenzhou Medical University, Wenzhou, China
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Dongsheng Yan
    State Key Laboratory of Ophthalmology, Wenzhou Medical University, Wenzhou, China
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Footnotes
    Commercial Relationships   Weiwei Chen, None; Shuai Yang, None; Zhonglou Zhou, None; Xiaoting Zhao, None; Dongsheng Yan, None
  • Footnotes
    Support  973 Project (2012CB722303) from the Ministry of Science and Technology of China, and Science Foundation of Wenzhou Medical University QTJ11020
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3857. doi:
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    • Get Citation

      Weiwei Chen, Shuai Yang, Zhonglou Zhou, Xiaoting Zhao, Dongsheng Yan; Identification and Characterization of Long Noncoding RNA Contributions to Mouse Corneal Development. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3857.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Long noncoding RNAs (lncRNAs) are important regulators of cellular functions. However an extensive in-depth analysis of their expression profile and function in mouse corneal development is still lacking. Here we investigated lncRNAs profiles of the developing mouse cornea by microarray and bioinformatics.

Methods : Affymetrix mouse transcriptome 1.0 assay identified corneal lncRNA profile changes occurring between birth and 8 weeks in mice. Real-time RT-PCR analysis validated array findings. Gene ontology (GO) and KEGG pathway mapping of protein-coding genes adjacent to signature lncRNAs clarified potential lncRNA roles in regulating corneal development.

Results : In newborn and 8-week-old mice, 41,987 protein-coding and noncoding gene transcripts were identified. In these two subsets, 19, 623 of this ensemble are lncRNAs annotated in public data banks. During development, 1,272 lncRNAs underwent ≧ two-fold changes in expression levels. qPCR analysis confirmed gene microarray analysis results since there was 90% agreement between the two procedures in identifying lncRNAs contributing to this process. GO analysis of protein-coding genes proximal to lncRNA signatures resolved numerous neighboring protein coding genes regulating cell division and adhesion as well as collagen and cytokine production, suggesting a role for signature lincRNAs in controlling corneal development.

Conclusions : Time dependent changes in lncRNA expression patterns occurring during mouse corneal development suggest that they play an important role in regulating this process.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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