September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Study on the effect of size of nanocarriers on trans-scleral transport
Author Affiliations & Notes
  • Rini Rachel Joseph
    Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Tina T Wong
    Singapore National Eye Centre, Singapore, Singapore, Singapore
    Ocular Drug Delivery Group, Singapore Eye Research Institute, Singapore, Singapore
  • Subbu Venkatraman
    Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Footnotes
    Commercial Relationships   Rini Joseph, None; Tina Wong, None; Subbu Venkatraman, None
  • Footnotes
    Support  NMRC/TCR/008/2013
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3989. doi:
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      Rini Rachel Joseph, Tina T Wong, Subbu Venkatraman; Study on the effect of size of nanocarriers on trans-scleral transport. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3989.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Nanocarriers have been used in drug delivery to increase the bioavailability of drug and to provide sustained delivery. Liposomes are particularly attractive for ocular drug delivery due to their excellent biocompatibility and non-toxicity. The aim of this work is to investigate the effect of liposome size on transcleral transport.

Methods : Multilamellar Vesicles (MLVs) were prepared using the thin film hydration technique. Size reduction of the MLVs was performed by subsequent extrusions to form Large Unilamellar Vesicles (LUVs) of desired sizes. Liposomes of different sizes were produced by varying the size of the filter used in extrusion. Porcine sclera was mounted in an Ussing chamber. The liposomes (~1µm and 90nm in size) were fluorescently tagged and used in the donor chamber of an ex vivo Ussing chamber setup, maintained at 37°C. Post transport study, the liposome localization in the tissue was visualized using an epifluorescence microscope.

Results : A size dependent transport behavior of the liposomes was observed over the study duration of 96 hours ex vivo. MLVs of approximately 1µm in size were found to remain on the episcleral surface and minimal liposome transport was observed through the sclera (average transport of 6.78% of total scleral thickness; SD =2.24%; n=3). LUVs of approximately 90nm were found to transport through a larger part of the sclera (Transport of 45.66% of the total scleral thickness; SD =0.95%; n=3).

Conclusions : This study shows that transcleral transport of liposomes is dependent on its size.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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