September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Inter-method agreement in retinal blood vessels diameter analysis between Dynamic Vessel Analyzer and Optical Coherence Tomography
Author Affiliations & Notes
  • Maria Vittoria Cicinelli
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Lucia Benatti
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Livia Tomasso
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Federico Corvi
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Andrea Mazzaferro
    Ophthalmology, University of Messina, Milan, Italy
  • Adriano Carnevali
    ophthalmology, University of Magna Graecia, Catanzaro, Italy
  • Ilaria Zucchiatti
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Lea Querques
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Francesco Bandello
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Giuseppe Querques
    Ophthalmology, San Raffaele Scientific Institute, Milan, Italy, Italy
  • Footnotes
    Commercial Relationships   Maria Vittoria Cicinelli, None; Lucia Benatti, None; Livia Tomasso, None; Federico Corvi, None; Andrea Mazzaferro, None; Adriano Carnevali, None; Ilaria Zucchiatti, None; Lea Querques, None; Francesco Bandello, None; Giuseppe Querques, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4222. doi:
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      Maria Vittoria Cicinelli, Lucia Benatti, Livia Tomasso, Federico Corvi, Andrea Mazzaferro, Adriano Carnevali, Ilaria Zucchiatti, Lea Querques, Francesco Bandello, Giuseppe Querques; Inter-method agreement in retinal blood vessels diameter analysis between Dynamic Vessel Analyzer and Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4222.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Measurement of retinal blood vessels diameter provides essential information about retinal involvement both in systemic disorders and ocular conditions. Recently, a method for blood vessel manual measurement and artery-to-vein ratio (AVR) calculation by means of optic nerve head-centered spectral domain optical coherence tomography (SD-OCT) has been proposed. Dynamic Vessel Analyzer (DVA; Imedos Systems, Ltd, Jena, Germany) allows automated AVR estimation through static analysis, and its software has been validated for retinal vessel assessment. Aim of this study is to analyze the inter-methods agreement in AVR evaluation between SD-OCT and DVA.

Methods : Healthy volunteers with no chronic systemic conditions or ocular disease were recruited from Department of Ophthalmology of University Scientific Institute San Raffaele in Milan, Italy. All participants underwent DVA and SD-OCT (Spectralis; Heidelberg Engineering, Heidelberg, Germany). Two 7-line cube scans were placed at the superior and inferior borders of optic disc, respectively. The mean diameter of the arteries and veins was manually calculated, twice, separately for each of the rasters by 2 independent readers (reader 1 and 2). SPSS statistics software version 20.0 (SPSS Inc, an IBM Company, Chicago, Illinois) was used for statistical analysis.

Results : Twenty-two eyes of 11 healthy subjects (5 women and 6 men, mean age 35) were included in the study. The mean AVR by DVA was 0.8036. Readers 1 and 2 showed high inter-observer agreement, but reader 1 showed lower intra-observer variability (mean1 0.9023 and mean2 0.9030, p=0.003) than reader 2 (mean1 0.9523 and mean2 0.9067, p=0.78), and therefore was chosen as reference. On this basis, AVR measured on SD-OCT turned out to be significally higher than AVR measured through DVA (p<0.001).

Conclusions : We demonstrated a significant difference in the two noninvasive methods for caliber measurement of retinal blood vessels, with a tendency of SD-OCT to overestimate retinal vascular caliber in comparison to DVA.
This comparison may be useful for achieving greater accuracy in evaluation of retinal vessel in ocular as well as systemic diseases. Further large-scale studies are needed to confirm our preliminary findings.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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