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Gisele Cristina Gonçalves de Almeida Cunha, Lucia ibares-Frias, Roberto Cantalapiedra, Patricia Gallego, María Carmen Martínez-García, Jesus Merayo-Lloves; UVAB and Cross Linking epi – off produce similar damage in hens cornea. Invest. Ophthalmol. Vis. Sci. 201657(12):.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the damage and wound healing in the hen animal model after UV – A and UV – B radiation and Crosslinking Procedure
Hens were randomly separated in 3 groups. The control group 1(n=8 eyes) did not receive treatment. Group 2 (n=20 eyes) were exposed to three doses of UV – A and UV – B radiation (4.60 J/cm2). Several clinical follow-ups were done during the essay and animals were euthanized 24 hours, day 15 and 30 after the last dose. The crosslinking group animals were called group 3 (20 eyes).. After deepithelization, they were exposure to 5.4 mJ/cm2 that corresponds to 3 mW/cm2. A clinical follow-up was done and the hens were euthanized 24 hours, day 15 and 30 after treatment. Corneas were stained with hematoxylin-eosin. Apoptosis was measured by terminal uridine nick end-labeling assays.
The UV – A and UV – B group radiation showed ulcers, corneal edema, hyperaemic conjunctiva. The histology showed a stromal edema and a reduction of the keratocytes number. The crosslinking groups showed hyperaemic conjunctiva and corneal edema. The number of keratocytes had reduced in the third top of the cornea. The edema and the epithelium disruption were subsided spontaneously few days after the both treatment finish
After the UV – A and UV – B radiation and crosslinking procedure, the number of keratocyes reduced drastically. This area is gradually recovered but the third top of the corneas was still free of keratocytes at day 15 and 30 after treatment. The results suggest that in spite of differences of treatments the UV – A could cause serious damages in the cornea.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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