September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Blueberry Component Pterostilbene Protects Corneal Epithelial Cells from Inflammatory and oxidative stress
Author Affiliations & Notes
  • Ding Chen
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
    School of Optometry and Ophthalmology, Wenzhou Medical University, Wenzhou, China
  • Jin Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
    School of Optometry and Ophthalmology, Wenzhou Medical University, Wenzhou, China
  • Ruzhi Deng
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
    School of Optometry and Ophthalmology, Wenzhou Medical University, Wenzhou, China
  • Xia Hua
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Lili Zhang
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Stephen C Pflugfelder
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • De-Quan Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Ding Chen, None; Jin Li, None; Ruzhi Deng, None; Xia Hua, None; Lili Zhang, None; Stephen Pflugfelder, None; De-Quan Li, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4353. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Ding Chen, Jin Li, Ruzhi Deng, Xia Hua, Lili Zhang, Stephen C Pflugfelder, De-Quan Li; Blueberry Component Pterostilbene Protects Corneal Epithelial Cells from Inflammatory and oxidative stress. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4353.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Pterostilbene (PS), a naturally dietary compound of blueberries, has been proven to be an anti-inflammatory and anti-oxidative agent. However, there is no study on its effect on ocular diseases, such as dry eye. This study was to explore potential protective effects of PS on dry eye using an in vitro culture model of human corneal epithelial cells (HCECs) exposed to hyperosmotic medium.

Methods : Primary HCECs were cultured from fresh donor limbal explants. Hyperosmolarity model was established by switching HCECs from isosmotic (312 mOsm) to hyperosmotic medium (450 mOsm) alone or in the presence of different concentration (5-20μM) of PS for 4-24h. Gene expression was detected by RT-qPCR; and protein production or activity was evaluated by ELISA, zymography, Western blotting and immunostaining. Reactive oxygen species (ROS) production was measured using a DCFDA kit.

Results : The addition of PS significantly reduced the expression of pro-inflammatory mediators, TNF-α, IL-1β, IL-6, MMP-2 and MMP-9 in HCECs exposed to hyperosmotic medium. Pre-treatment with PS (5 to 20µM) suppressed ROS overproduction in a dose-dependent manner. Additionally, PS significantly decreased the levels of oxidative damage biomarkers, malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), aconitase-2 and 8-hydroxydeoxyguanosine (8-OHdG). Importantly, PS was found to rebalance homeostasis between oxygenases and anti-oxidative enzymes by decreasing cyclooxygenase 2 (COX2) expression and restoring the activity of antioxidant enzymes, super oxide dismutase 1 (SOD1) and peroxiredoxin-4 (PRDX4) during hyperosmotic stress.

Conclusions : Our findings demonstrate for the first time that blueberry component pterostilbene protects the human cornea from hyperosmolarity-induced inflammation and oxidative stress, suggesting the protective potential to dry eye disease.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×