September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Comparison of conjunctival scarring after microincision vitrectomy surgery using 20-, 23-, 25-, and 27-gauge systems in rabbits
Author Affiliations & Notes
  • Makoto Gozawa
    Ophthalmology, University of Fukui, Yoshida Fukui, Fukui, Japan
  • Yoshihiro Takamura
    Ophthalmology, University of Fukui, Yoshida Fukui, Fukui, Japan
  • Seiji Miyake
    Ophthalmology, University of Fukui, Yoshida Fukui, Fukui, Japan
  • Kentaro Iwasaki
    Ophthalmology, University of Fukui, Yoshida Fukui, Fukui, Japan
  • Shogo Arimura
    Ophthalmology, University of Fukui, Yoshida Fukui, Fukui, Japan
  • Yuji Takihara
    Ophthalmology, University of Fukui, Yoshida Fukui, Fukui, Japan
  • Masaru Inatani
    Ophthalmology, University of Fukui, Yoshida Fukui, Fukui, Japan
  • Footnotes
    Commercial Relationships   Makoto Gozawa, None; Yoshihiro Takamura, None; Seiji Miyake, None; Kentaro Iwasaki, None; Shogo Arimura, None; Yuji Takihara, None; Masaru Inatani, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4449. doi:
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    • Get Citation

      Makoto Gozawa, Yoshihiro Takamura, Seiji Miyake, Kentaro Iwasaki, Shogo Arimura, Yuji Takihara, Masaru Inatani; Comparison of conjunctival scarring after microincision vitrectomy surgery using 20-, 23-, 25-, and 27-gauge systems in rabbits. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4449.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare conjunctival scarring after vitrectomy in rabbit eyes using different gauge systems by analyzing anterior segment optical coherence tomography (AS-OCT) images and histological sections.

Methods : Vitrectomy using 20-, 23-, 25-, and 27-gauge systems was performed for rabbits. AS-OCT images of the incision sites were obtained before and at day 1, 7 and 1 month after surgery. We measured the thickness of the conjunctiva and calculated the preservation rates of the borderlines among the subconjunctival layers. Surgical invasion was estimated by histological observation and immunohistological staining for α-smooth muscle actin.

Results : The total conjunctiva and conjunctival stroma/Tenon’s capsule in the 20-gauge group were significantly thicker than that in the 27-gauge group at day 1 and day 7 after surgery. Preservation rates of the conjunctival stroma/Tenon’s capsule borderline were significantly lower in the 20-gauge group than in the 25- and 27-gauge groups at day 1, day 7, and 1 month. Preservation rates of the Tenon’s capsule/sclera borderline were significantly lower in the 20-gauge group than in the 25- and 27-gauge groups at 1 month. In the 27-gauge group, the number of α-smooth muscle actin-positive fibroblasts was significantly smaller than in the 20-gauge group at day 7.

Conclusions : Based on the finding of AS-OCT and histology, micro incision vitreous surgery, especially using 27-gauge, contributed to less conjunctival scarring postoperatively. Therefore, the 27-gauge pars plana vitrectomy may be a more effective technique for preserving the structure of conjunctiva.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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