September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Adiponectin protects against hyperglycemia-induced retinal abnormalities in retinopathy of prematurity
Author Affiliations & Notes
  • Zhongjie Fu
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Raffael Liegl
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Nicholas Saba
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Peyton Morss
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Thomas Fredrick
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Steven Meng
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Samuel Burnim
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Jing Chen
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Ann Hellstrom
    Ophthalmology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
  • Lois E H Smith
    Ophthalmology, Children's Hosp Boston/Harvard Med Sch, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Zhongjie Fu, None; Raffael Liegl, None; Nicholas Saba, None; Peyton Morss, None; Thomas Fredrick, None; Steven Meng, None; Samuel Burnim, None; Jing Chen, None; Ann Hellstrom, None; Lois Smith, None
  • Footnotes
    Support  NIH/NEI (R01 EY022275, R01 EY017017, P01 HD18655), Lowy Medical Foundation, European Commission FP7 (305485) (LEHS); Knights Templar Eye Foundation, The Bernadotte Foundation (ZF)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4525. doi:
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      Zhongjie Fu, Raffael Liegl, Nicholas Saba, Peyton Morss, Thomas Fredrick, Steven Meng, Samuel Burnim, Jing Chen, Ann Hellstrom, Lois E H Smith; Adiponectin protects against hyperglycemia-induced retinal abnormalities in retinopathy of prematurity. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4525.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Retinopathy of prematurity (ROP) is a blinding eye disorder in premature infants. Hyperglycemia is correlated with all stages especially proliferative ROP as well as with decreased serum adiponectin (APN) levels. Adipocyte-derived APN influences glucose and lipid metabolism in many tissues and serum APN levels correlate with postnatal growth and gestational age. Full-term newborns have higher APN levels in cord blood than preterm infants. We previously showed that in preterm infants, lower serum APN levels correlate with increased ROP development. We aimed to determine if APN protects against the hyperglycemia-induced retinal abnormalities in ROP.

Methods : APN-/- (KO) and wild-type (WT) mice were treated with streptozotocin (STZ) to induce hyperglycemia. In the mouse model of STZ-induced hyperglycemia in oxygen-induced retinopathy (STZ/OIR), at P17, blood glucose levels were recorded; retinal vaso-obliteration and neovascularization (NV) were quantified. Electroretinography was examined at P30. Ex vivo choroidal assay was conducted. Retinal notch signaling in normal and neovascular blood vessels was examined with laser-captured microdissection.

Results : In STZ/OIR, the combination of hyperglycemia and APN deficiency exacerbated retinal NV (APN-/--STZ/WT-control=2.4; WT-STZ/WT-control=1.5; APN-/--control/WT-control=1.5). Hyperglycemia attenuated the sensitivity of a-wave, sensitivity and amplitude of b-wave as well as the summed oscillatory potentials amplitude. Hyperglycemia inhibited ex vivo choroidal microvascular sprouting especially with APN deficiency. Reduced expression of notch 1 to 4 was observed in neovessels at P17.

Conclusions : Hyperglycemia worsens retinopathy in neonatal mice and APN deficiency exacerbates hyperglycemic retinopathy, suggesting that APN might be a potential therapeutic target for the treatment of hyperglycemia-associated retinal abnormalities.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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