September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Ca2+ activity during ATP-induced relaxation of porcine retinal arterioles in vitro spreads along the processes of the perivascular cells
Author Affiliations & Notes
  • Olga Kudryavtseva
    Ophthalmology, Aarhus Uiversity Hospital, Aarhus, Denmark
  • Toke Bek
    Ophthalmology, Aarhus Uiversity Hospital, Aarhus, Denmark
  • Footnotes
    Commercial Relationships   Olga Kudryavtseva, None; Toke Bek, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4536. doi:
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      Olga Kudryavtseva, Toke Bek; Ca2+ activity during ATP-induced relaxation of porcine retinal arterioles in vitro spreads along the processes of the perivascular cells. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4536.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Recently, a novel population of perivascular cells (PVC) located immediately external to the vascular smooth muscle cells of retinal arterioles has been identified. These cells display Ca2+ activity simultaneously with relaxation of retinal arterioles and are connected to cellular processes extending into the retinal tissue. It has also been shown that retinal arterioles with intermediate contraction have a biphasic response to ATP with an initial contraction followed by a relaxation. The aim of this study was to investigate Ca2+ signals in PVCs and their process during successive contraction and relaxation of retinal arterioles induced by ATP.

Methods : Porcine retinal arterioles with preserved perivascular retinal tissue were mounted in a confocal myograph and loaded with the Ca2+-sensitive fluorophore Oregon Green. The arterioles were preconstricted with 10-7 – 10-6 M U46619, and after the addition of ATP (10-4 M) vascular tone was recorded in a wire myograph and intracellular fluorescence in a laser scanning confocal microscope.

Results : The addition of ATP induced Ca2+ waves in PVCs (n=32) and their processes (n=7) both during contraction and relaxation of retinal arterioles. There was no significant difference between the frequency of Ca2+ waves in PVCs and their processes during contraction and relaxation (p>0.05), but the amplitude of Ca2+ waves was significantly higher (p<0.01) during contraction than during relaxation in the two structures.

Conclusions : The amplitude of Ca2+ waves in perivascular cells and their processes might potentially be used to differentiate contracting and relaxing responses of retinal arterioles.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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