September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Differential effect of prostaglandins on γ-glutamyltranspeptidase activity and glutamate/cystine uptake in trabecular meshwork cells.
Author Affiliations & Notes
  • Omar Shoukfeh
    Ophthalmology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States
  • Chanping Liang
    Ophthalmology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States
  • Marlyn P Langford
    Ophthalmology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States
  • Footnotes
    Commercial Relationships   Omar Shoukfeh, None; Chanping Liang, None; Marlyn Langford, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4692. doi:
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      Omar Shoukfeh, Chanping Liang, Marlyn P Langford; Differential effect of prostaglandins on γ-glutamyltranspeptidase activity and glutamate/cystine uptake in trabecular meshwork cells.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4692.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the effects of prostaglandin (PG) treatment on γ-glutamyltranspeptidase (GGT; plays key extracellular role in recapture of Cys for glutathione synthesis) activity and glutamate (Glu) and cystine (Cys) uptake in trabecular meshwork cells (TMC).

Methods : The effect of PG treatment on TMC GGT activity was determined by standard GGT assay utilizing conversion of γ-glutamyl p-nitroanilide in the presence of glycyl-gylcine with or without acivicin (AT125, GGT inhibitor). The Na+-independent uptake of radiolabeled Cys and Glu by Xc- antiporter was determined in replicate TMC cultures treated with different concentrations of PG A2, D2, F1 and F2.

Results : TMC GGT activity was inhibited 50±16% by 10-300 ng/mL PGF1 and AT125. GGT activity was inhibited 23-41% by 1 ug/mL of PGA2, D2 or F2. Concomitantly, PGA2, and PGF1 treatment of TMC did not significantly affect Na+-independent uptake of radiolabeled Glu or Cys. However, PGD2 increased Na+-independent radiolabeled Glu uptake by 39±11%, but not Cys uptake (<2.5%). In contrast, PGF2 treatment induced a dose-dependent decrease in TMC Na+-independent radiolabeled Glu uptake (up to 37±9%) and radiolabeled Cys uptake (up to 12±7%).

Conclusions : TMC GGT and Xc- antiporter activity can be modulated by prostaglandins. The results suggest that endogenous and exogenous prostaglandins may regulate TMC function in vivo via modulation of GGT and Xc- antiporter coordinated activities.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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