September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Antifungal defence proteins induced in tear fluid of mycotic keratits patients – A quantitative proteomics study
Author Affiliations & Notes
  • Dharmalingam Kuppamuthu
    Aravind Medical Research Foundation, Madurai, India
  • Jeyalakshmi Kandhavelu
    Aravind Medical Research Foundation, Madurai, India
  • Naveen Luke Demonte
    Aravind Medical Research Foundation, Madurai, India
  • Chitra Thangavel
    Aravind Medical Research Foundation, Madurai, India
  • Jeya Maheshwari Jayapal
    Aravind Medical Research Foundation, Madurai, India
  • Lalitha Prajna
    Department of Ocular Microbiology, Aravind Eye Hospital, Madurai, India
  • Venkatesh Prajna Namperumalsamy
    Cornea Clinic, Aravind Eye Hospital, Madurai, India
  • Footnotes
    Commercial Relationships   Dharmalingam Kuppamuthu, None; Jeyalakshmi Kandhavelu, None; Naveen Demonte, None; Chitra Thangavel, None; Jeya Maheshwari Jayapal, None; Lalitha Prajna, None; Venkatesh Prajna Namperumalsamy, None
  • Footnotes
    Support  DBT Grant BT/01/CEIB/11/VI/09; MindTree Grant
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4810. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Dharmalingam Kuppamuthu, Jeyalakshmi Kandhavelu, Naveen Luke Demonte, Chitra Thangavel, Jeya Maheshwari Jayapal, Lalitha Prajna, Venkatesh Prajna Namperumalsamy; Antifungal defence proteins induced in tear fluid of mycotic keratits patients – A quantitative proteomics study. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4810.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To examine the tear fluid for the presence of host defence proteins induced by infecting pathogenic filamentous fungi, A. flavus.

Methods : Tear fluid was collected from A.flavus keratitis patients during the early stage of infection using capillary tubes. Pooled tear samples were fractionated using lectin affinity chromatography and/or by 1D SDS-PAGE. Proteins were then subjected to tryptic digestion and taken for identification using Orbitrap Velos Pro mass spectrometer. iSRM analysis of selected proteins were done using triple quadrupole mass spectrometer.

Results : Proteins that show variable levels indicate the activation of all the three complement pathways, antifungal neutrophil defence in the form of NETosis, along with inflammatory and wound healing responses. Proteins indicative of these processes are found at higher levels in tear from keratitis patients. Since these observations are made using early stage tear, presumably proteins secreted by the fungus or the fungal surface proteins could be responsible for this host response. Functional role of these proteins will be presented.

Conclusions : Activation of pathways involved in defence against invading pathogens in the early stage of infection suggests that the host generates a robust antifungal defence. Despite this, a subset of the keratitis patients are still susceptible to fungal infection. This could probably be due to the fungal induced inhibitors that could either directly or indirectly (by activating inhibitory host response) attenuate the robust host response against infection.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×