September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Gangliosides in the retina: a comparative inventory with brain and other ocular structures
Author Affiliations & Notes
  • Elodie Masson
    Eye and Nutrition Research Group, CSGA, 1324 INRA- 6265 CNRS- Bourgogne Franche-Comté University, DIJON, France
  • Estelle Sibille
    ChemoSens platform, CSGA, 1324 INRA- 6265 CNRS- Bourgogne Franche-Comté University, Dijon, France
  • Lucy Martine
    Eye and Nutrition Research Group, CSGA, 1324 INRA- 6265 CNRS- Bourgogne Franche-Comté University, DIJON, France
  • Olivier Berdeaux
    ChemoSens platform, CSGA, 1324 INRA- 6265 CNRS- Bourgogne Franche-Comté University, Dijon, France
  • Alain M Bron
    Dept of Ophtalmology, Dijon University Hospital, Dijon, France
  • Catherine P Garcher
    Dept of Ophtalmology, Dijon University Hospital, Dijon, France
  • Zhiguo He
    Laboratory "Biology, Imaging, and Engineering of Corneal Grafts" EA2521, University Jean Monnet, Saint-Etienne, France
  • Gilles Thuret
    Laboratory "Biology, Imaging, and Engineering of Corneal Grafts" EA2521, University Jean Monnet, Saint-Etienne, France
  • Lionel Bretillon
    Eye and Nutrition Research Group, CSGA, 1324 INRA- 6265 CNRS- Bourgogne Franche-Comté University, DIJON, France
  • Footnotes
    Commercial Relationships   Elodie Masson, None; Estelle Sibille, None; Lucy Martine, None; Olivier Berdeaux, None; Alain Bron, None; Catherine Garcher, None; Zhiguo He, None; Gilles Thuret, None; Lionel Bretillon, None
  • Footnotes
    Support  National Institute for Research on Agronomy (INRA); Regional Council of Burgundy; European Regional Development Fund (FEDER)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4812. doi:
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      Elodie Masson, Estelle Sibille, Lucy Martine, Olivier Berdeaux, Alain M Bron, Catherine P Garcher, Zhiguo He, Gilles Thuret, Lionel Bretillon; Gangliosides in the retina: a comparative inventory with brain and other ocular structures. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4812.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Gangliosides (GG) make an extremely diverse family of glycosphingolipids particularly abundant in the brain and neural tissues. While it is known that alterations in GG metabolism are associated with visual defects, the precise biological roles of these compounds in the retina are still largely unknown. In this context, we performed an exhaustive inventory and thorough characterization of the human eye GG aiming to reveal specificities and common features of the retina compared to other ocular tissues and brain.

Methods : Retina, RPE/Choroid, ciliary body, optic nerve and brain samples were collected from deceased human donors (N=7). GG were extracted and analysed by a comprehensive approach relying on various techniques. First, GG content and class composition were assessed by high-performance thin layer chromatography followed by colorimetric revelation. Second, identification and relative quantification of the different ceramide types represented in each GG class were achieved using liquid chromatography coupled to mass spectrometry. Finally, high-resolution mass spectrometry allowed for the identification of original GG classes and structural characterization of the ceramide moieties (sphingoïd base and fatty acid)

Results : Retina contained the highest amount of GG among the ocular tissues tested, although much less than brain. It also presented with a wide diversity of GG classes. As other types of nervous tissues, the retina contained high levels of tetraosylGG but the prominence of GD3, GT3 and acetylated GT3 appeared as a specificity of this tissue. Retina also exhibited the largest diversity of ceramide types sharing common features with brain. They both had especially high proportions of monounsaturated ceramides and consecutively low proportions of polyunsaturated ceramides, as well as low proportions of ceramides shorter than 36 carbons or longer than 41 carbons. Moreover, GG of the retina contained 4 different types of sphingoïd bases: mostly d18:1 as every other tissues but also significant levels of d20:1 as the brain and optic nerve and in minor proportions d16:1 and d18:2 also found in ciliary body.

Conclusions : Our observations show that the retina exhibits a specific GG signature compared to other ocular tissues or to the brain suggesting a particular role of some GG classes or ceramide species in relation to the unique structure and function of this highly specialised tissue.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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