September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Cross-linking and photoablative keratectomy
Author Affiliations & Notes
  • Jean-Louis Bourges
    Department of Ophthalmology, Hopitaux Universitaires Paris Centre, Hôtel-Dieu, Assistance Publique-Hôpitaux de Paris, Paris, France
    Paris Descartes Faculty of medicine, INSERM UMR 1138, team 17, Sorbonne Paris Cité university, Paris, France
  • Chadi Mehana
    Department of Ophthalmology, Hopitaux Universitaires Paris Centre, Hôtel-Dieu, Assistance Publique-Hôpitaux de Paris, Paris, France
  • Laurent Jonet
    Paris Descartes Faculty of medicine, INSERM UMR 1138, team 17, Sorbonne Paris Cité university, Paris, France
  • Michèle Savoldelli
    Paris Descartes Faculty of medicine, INSERM UMR 1138, team 17, Sorbonne Paris Cité university, Paris, France
  • Christophe Panthier
    Department of Ophthalmology, Hopitaux Universitaires Paris Centre, Hôtel-Dieu, Assistance Publique-Hôpitaux de Paris, Paris, France
  • Francine F Behar-Cohen
    department of ophthalmology, Université de Lausanne, Jules Gonin Hospital FAA, Lausanne, Switzerland
    Paris Descartes Faculty of medicine, INSERM UMR 1138, team 17, Sorbonne Paris Cité university, Paris, France
  • Footnotes
    Commercial Relationships   Jean-Louis Bourges, None; Chadi Mehana, None; Laurent Jonet, None; Michèle Savoldelli, None; Christophe Panthier, None; Francine Behar-Cohen, None
  • Footnotes
    Support  Support provided: Banque Francaise des Yeux graciously provided experimental corneas and Abbott Medical Optics-Laser division graciously provided laser procedures
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4853. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jean-Louis Bourges, Chadi Mehana, Laurent Jonet, Michèle Savoldelli, Christophe Panthier, Francine F Behar-Cohen; Cross-linking and photoablative keratectomy. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4853.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Refractive or therapeutic photoablations have been proposed in association with collagen cross-linking (CXL) protocols, despite riboflavin masks part of ultraviolet (UV) at excimer’s wavelengths. We aimed therefore at investigating photoablation depths resulting from excimer laser treatment on human corneas exposed to Riboflavin and UV.

Methods : Human experimental corneas were obtained from the Banque Francaise des Yeux. Experiments were conducted in the department of ophthalmology at Hotel-Dieu hospital, Paris.
We separated experimental corneas in 3 groups of treatment: Group BSS (n=3) exposed to BSS only, group B2 (n=3) exposed to Riboflavin and group B2-UV (n=4) exposed to Riboflavin then irradiated with UV 10 mW/mm2-9 min. All cornea were then subjected to a myopic 4 D PRK (intended 61 µm depth). Corneas were photoablated on half of a 6.5mm central area, by separating the optic zone (OZ) with a PMMA plate. The central corneal thickness (CCT) was measured by US pachymetry before BSS/Riboflavin exposure, and after PRK. Corneas were then fixed and processed for optical microscopy. The maximal depth of ablation was quantified on semithin section in each group at the ablation transition line within a 1 mm central area.

Results : Mean (±SD) CCTs of Group BSS, B2 and B2-UV were initially 628±3 µm, 729±2 µm and 665±1 µm. All CCTs decreased after exposure and before PRK from 12%, 20% and 37% respectively. Mean photoablation depths (±SD) were BSS=113±30 µm, B2=103±46 µm and B2+UV=105±13 µm.

Conclusions : Corneal edema interferes with excimer laser by enhancing depth of photoablation. The full collagen cross-linking procedure seems to limit PRK mean photoablation depths compared to BSS or Riboflavin alone.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×