September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
CELL BEHAVIOR OF CORNEAL TISSUE AFTER REFRACTIVE SURGERY WITH TECHNIQUE SMILE, BY CONFOCAL MICROSCOPY.
Author Affiliations & Notes
  • Martha Paulina Gonzalez
    STUDENT, Hospital de la Luz (FHNSL), Distrito Federal, Mexico State, Mexico
  • Oscar Fernandez
    CORNEA, Hospital de la Luz (FHNSL), MEXICO, Mexico State, Mexico
  • Footnotes
    Commercial Relationships   Martha Gonzalez, None; Oscar Fernandez, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4892. doi:
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      Martha Paulina Gonzalez, Oscar Fernandez; CELL BEHAVIOR OF CORNEAL TISSUE AFTER REFRACTIVE SURGERY WITH TECHNIQUE SMILE, BY CONFOCAL MICROSCOPY.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4892.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Analyze cell behavior of corneal tissue after refractive surgery with SMILE,in the period of January to May 2015, by confocal microscopy.

Methods : Prospective,observational,descriptive and longitudinal study of fifty two eyes.Corneal tissue was analyzed during 6° months,taking an area 0.0192mm2 with NAVIS3 program evaluating basal cell active keratocytes in the anterior and posterior to the surgical area interface and endothelial cell count with the Kruskal Wallis test.Subbasal presence and degree of reflectivity surgical interface (severe,moderate and mild) nerve plexus was sought.

Results : The average basal cells in the basal state was 4783 cells / mm2, in the first days post-surgery was 5214 cells / mm2,as of 5040 cells/mm2,the third month of 5173 cells/mm2,and the 6th month 5243 cells/mm2,no significant changes (p =0.4060) were;no change in morphology was observed during follow-up.The subbasal nerve plexus observed in some eyes to the first analyzed month, but after the third month and is displayed on the 72.5% of the population .In anterior stroma an overall average of 21 keratinocytes by area, of which 13 assets were observed in the first fifteen days, by month 12 keratinocytes, the third month 7 keratinocytes and keratinocytes 4 active sixth month, showing in this way was obtained progressive decrease in metabolic activity thereof. In posterior stroma, a total average of 15 keratinocytes was obtained by area; of which 4 active keratinocytes in the first 15 days, a month 5, the third month 3 keratinocytes, and the sixth month 2 active keratinocytes.Surgical interface presents intense reflectivity early postoperative (57.6%) and during their evolution tends to reduce reflectivity showing a mild to moderate from the third month to the sixth month..

Conclusions : The evaluated corneal tissue cells suffered no permanent morphological changes, tending to return to its ground state. The subbasal nerve tissue is attacked not fully allowing the progressive regeneration of the same after the third month.Surgical interface reflectivity occurs most in the first few months, but with the course of analysis persistence of scar tissue,but the reflectivity tends to decline slowly appreciated. In endothelial cell movement does not exist during the study period.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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