September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Role of microRNA-145 in pterygium cell apoptosis
Author Affiliations & Notes
  • Di CAO
    Ophthalmology and Visual Sciences, The Chinese University Of Hong Kong, Hong Kong , China
  • Tsz Kin Ng
    Ophthalmology and Visual Sciences, The Chinese University Of Hong Kong, Hong Kong , China
  • Yufei Teng
    Beijing Tongren Hospital, Capital Medical University, BeiJing, China
  • Wong Ying Yip
    Ophthalmology and Visual Sciences, The Chinese University Of Hong Kong, Hong Kong , China
  • Alvin Young
    Ophthalmology and Visual Sciences, The Chinese University Of Hong Kong, Hong Kong , China
  • Chi Pui Calvin Pang
    Ophthalmology and Visual Sciences, The Chinese University Of Hong Kong, Hong Kong , China
  • Vishal Jhanji
    Ophthalmology and Visual Sciences, The Chinese University Of Hong Kong, Hong Kong , China
  • Footnotes
    Commercial Relationships   Di CAO, None; Tsz Kin Ng, None; Yufei Teng, None; Wong Ying Yip, None; Alvin Young, None; Chi Pui Pang, None; Vishal Jhanji, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 4928. doi:
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      Di CAO, Tsz Kin Ng, Yufei Teng, Wong Ying Yip, Alvin Young, Chi Pui Calvin Pang, Vishal Jhanji; Role of microRNA-145 in pterygium cell apoptosis. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4928.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Downregulation of microRNA-145 (miRNA-145) has been correlated with increased severity of pterygium. We hypothesized that forced expression of miRNA-145 could attenuate pterygium growth.

Methods : Ectopic expression of miRNA-145 in primary pterygium cells was achieved by transfection of miRNA-145 mimics. Cell properties of miRNA-145-transfected pterygium cells were analyzed by MTT assay, migration assay and flow cytometry. The expression of miRNA-145-related targets, mouse double minute 2 (MDM2) and p53, was determined by flow cytometry and immunoblotting.

Results : Pterygium cells transfected with miRNA-145 mimics showed lower cell viability (5.71 folds, p<0.001) and retarded migration (71.78% vs 82.10%, p<0.05), compared to those transfected with scramble control. Moreover, the proportion of sub-G1 phase in miRNA-145-transfected cells (40.9%) was higher than that in scramble control (7.32%, p < 0.05). Flow cytometry and immunoblotting analyses confirmed that MDM2 expression was reduced in miRNA-145-transfected pterygium cells compared to scramble control. In contrast, the expression of p53, which is negatively regulated by MDM2, was increased after miRNA-145 transfection.

Conclusions : This study demonstrated that ectopic expression miRNA-145 reduces pterygium cell viability and migration as well as induce cell apoptosis. MDM2 was downregulated, whereas p53 was upregulated by miRNA-145.. Our results suggest that overexpression of miRNA-145 in pterygium cells could be a potential therapeutic strategy for pterygium treatment through MDM2/p53-induced apoptotic pathway.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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