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Takeshi Yoshida, Tomoka Ishida, Kosei Shinohara, Ikuo Morita, Kyoko Ohno-Matsui; The correlation between sirt1 and Muller cells in the development of CNV. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4989.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate a correlation with Muller cells and sirtuin 1 (sirt1) in a development of choroidal neovascularization (CNV).
We developed laser induced choroidal neovascularization (CNV) in C57/BL6 mice. And then the eyes were applied intravitreal injection of sirt1 activator, Resveratrol (RSV; 0 and 30µM) at same day. After 1, 3, 5 and 7 days from the application, the eyes were sampled and used for immunohistochemistry of glial fibrillary acidic protein (GFAP: marker of activated Muller cell) (n=4 each). Furthermore, we made whole mount samples of the eyes and immunostained with isolectin B4 (IB4) at day 7 from the application (n=10). Finally, we analyzed the volume of CNV using confocal microscopy and Imaris software.
The GFAP(+) cells were observed around the experimental CNV area. We revealed that intravitreal injection of RSV promoted the GFAP(+) cells migration into CNV region from day 5. However, the GFAP(+) cells migration was not observed until day 7 in control eyes. And the intravitreal injection of RSV significantly decreased the CNV volume by 33% comparing to control at day 7 (p<0.05).
Our results showed sirt1 activation could modulate muller cells activation, which may have an inhibitory effect on CNV development. Muller cells may become a new therapeutic target for treatment of CNV.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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