September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Four-dimensional imaging revealed influences of monocyte chemoattractant protein-1 on immune cell dynamics in the subconjunctival tissue.
Author Affiliations & Notes
  • Sachi Kojima
    Ophthalmology, Kumamoto University, Kumamoto, Kumamoto, Japan
  • Toshihiro Inoue
    Ophthalmology, Kumamoto University, Kumamoto, Kumamoto, Japan
  • Tomokazu Fujimoto
    Ophthalmology, Kumamoto University, Kumamoto, Kumamoto, Japan
  • Hidenobu Tanihara
    Ophthalmology, Kumamoto University, Kumamoto, Kumamoto, Japan
  • Footnotes
    Commercial Relationships   Sachi Kojima, None; Toshihiro Inoue, None; Tomokazu Fujimoto, None; Hidenobu Tanihara, None
  • Footnotes
    Support  JSPS KAKENHI 15K15636
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5148. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Sachi Kojima, Toshihiro Inoue, Tomokazu Fujimoto, Hidenobu Tanihara; Four-dimensional imaging revealed influences of monocyte chemoattractant protein-1 on immune cell dynamics in the subconjunctival tissue.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5148.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To visualize influences of monocyte chemoattractant protein-1 (MCP-1) on the immune cells in the subconjunctival tissue using two-photon laser scanning microscope.

Methods : LysM-eGFP mice (gene-targeted mice expressing enhanced green fluorescent protein under the control of the endogenous lysozyme M promoter) were anesthetized by isoflurane. Vessels were visualized by intravenous injection of 70 kDa of Texas red–conjugated dextran. Using two-photon laser scanning microscope, three-dimensional images of the subconjunctival tissue were acquired every 1 minute for 30 minutes through phosphate-buffered saline with or without 10µg/ml MCP-1 in custom-made eyecup. Raw imaging data were processed and analyzed with Imaris software.

Results : Intravital green-labeled LysM-eGFP-positive cells and red-labeled vessels were successfully visualized using two-photon laser scanning microscope. Compared with the control, the number of LysM-eGFP-positive cells in the subconjunctival tissue increased in the presence of MCP-1. Moreover, the motility LysM-eGFP-positive cells was enhanced under the influence of MCP-1.

Conclusions : Four-dimensional imaging using two-photon microscope indicated MCP-1 affected the cellular dynamics in the subconjunctival tissue. The evaluation of cellular dynamics in the subconjunctival tissue using this technique may lead to the understanding of the wound healing process after filtration surgery.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×