September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Characterization of hiPSC-RPE on a prosthetic Bruch’s Membrane
Author Affiliations & Notes
  • Ruchira Singh
    Department of Ophthalmology (Flaum Eye Institute) and Biomedical Genetics , University of Rochester, Rochester, New York, United States
    Center for Visual Science, University of Rochester, Rochester, New York, United States
  • Audra Shadforth
    Queensland Eye Institute, South Brisbane , Queensland, Australia
    Queensland University of Technology, Brisbane, Queensland, Australia
  • Chad Galloway
    Department of Ophthalmology (Flaum Eye Institute) and Biomedical Genetics , University of Rochester, Rochester, New York, United States
  • Ali Hashim
    Department of Ophthalmology (Flaum Eye Institute) and Biomedical Genetics , University of Rochester, Rochester, New York, United States
  • Leslie MacDonald
    Department of Ophthalmology (Flaum Eye Institute) and Biomedical Genetics , University of Rochester, Rochester, New York, United States
  • Shuko Suzuki
    Queensland Eye Institute, South Brisbane , Queensland, Australia
  • Damien Gerard Harkin
    Queensland Eye Institute, South Brisbane , Queensland, Australia
    Queensland University of Technology, Brisbane, Queensland, Australia
  • Footnotes
    Commercial Relationships   Ruchira Singh, None; Audra Shadforth, None; Chad Galloway, None; Ali Hashim, None; Leslie MacDonald, None; Shuko Suzuki, None; Damien Harkin, None
  • Footnotes
    Support  Brightfocus Foundation Macular Degeneration Research Grant, David Bryant Trust, Knight Templar Eye Foundation Research Starter Grant, Research to prevent Blindness (Career Development Award, Singh-PI), Research to Prevent Blindness (unrestricted grant, Feldon- PI) Retina Research Foundation Pilot Study Grant, University Research Award (University of Rochester).
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5322. doi:
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      Ruchira Singh, Audra Shadforth, Chad Galloway, Ali Hashim, Leslie MacDonald, Shuko Suzuki, Damien Gerard Harkin; Characterization of hiPSC-RPE on a prosthetic Bruch’s Membrane. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5322.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The Bruch’s membrane is a multilayered collagenous structure, positioned at the basal side of the retinal pigment epithelium (RPE) cells, immediately apical to the choroidal vasculature in the eye. Disruption of the integral intra-cellular communication and physiologic interaction between these cell layers results in the thickening of this membrane in both genetic and age related macular degenerative diseases. It’s positioning in the eye makes it an obligate component of any model investigating the pathophysiology of the RPE/choroid complex ex vivo. The goal of this study was to evaluate the utility of a Bombyx mori silk fibroin (BMSF) membrane to serve as a prosthetic Bruch’s membrane.

Methods : Human inducible pluripotent stem cells (hiPSC)-derived RPE or human fetal RPE (hfRPE), were cultured either directly on BMSF membrane or extracellular matrix (ECM)-coated BMSF membrane. After 60-90 days in culture, morphologic, gene/protein expression and functional characteristics of hiPSC-RPE and hfRPE on BMSF membrane were compared to hiPSC-RPE and hfRPE grown on ECM coated plastic/permeable support transwells that have previously been shown to display important characteristics of in vivo adult human RPE.

Results : Both hiPSC and hfRPE grew on BMSF membranes in culture with varied efficiency dependent on the matrix coating of the membrane. Furthermore, hiPSC-RPE and hfRPE grown on ECM-coated BMSF membrane supported RPE growth and pigmentation while BMSF alone displayed poor adhesion. Furthermore, hiPSC-RPE cultured on matrix-coated BMSF displayed numerous physical, gene/protein expression and functional characteristics akin of in vivo human adult RPE cultures.

Conclusions : The BMSF membrane is sufficient to support the long-term culture of RPE from both fetal and hiPSC origins and will provide a suitable Bruch’s membrane-like platform for in vitro disease modeling and in vivo cell-transplantation studies.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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