September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Increased ocular levels of microRNA-148a in cases of retinal detachment promote epithelial–mesenchymal transition
Author Affiliations & Notes
  • Kei Takayama
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Hiroki Kaneko
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Fuxiang Ye
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Shiang-Jyi Hwang
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Takeshi Iwase
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Tetsu Asami
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Yasuki Ito
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Shinji Ueno
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Shunsuke Yasuda
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Hiroko Terasaki
    Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • Footnotes
    Commercial Relationships   Kei Takayama, None; Hiroki Kaneko, None; Fuxiang Ye, None; Shiang-Jyi Hwang, None; Takeshi Iwase, None; Tetsu Asami, None; Yasuki Ito, None; Shinji Ueno, None; Shunsuke Yasuda, None; Hiroko Terasaki, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5364. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Kei Takayama, Hiroki Kaneko, Fuxiang Ye, Shiang-Jyi Hwang, Takeshi Iwase, Tetsu Asami, Yasuki Ito, Shinji Ueno, Shunsuke Yasuda, Hiroko Terasaki; Increased ocular levels of microRNA-148a in cases of retinal detachment promote epithelial–mesenchymal transition. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5364.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The purpose of this study was to determine microRNA expression in vitreous and sub-retinal fluid (SRF) samples from patients with retinal detachment (RD). The pathological importance of the identified microRNA transcript levels was analyzed in vitro.

Methods : Vitreous fluid was collected from 10 patients with macular hole (MH), vitreomacular traction syndrome (VMTS), or foveoschisis and from 11 patients with RD. SRF was collected from 7 patients with RD. Of these, blood serum was collected in 4 patients. MicroRNA microarray profiling was performed to identify microRNA transcripts that were present in vitreous fluid, and more redundantly detected in SRF, from the patients with RD, but not detected in control eyes. Western blotting and scratch assays were performed in ARPE-19 cells and primary human RPE cell lines transfected with microRNA to elucidate the effect of identified microRNA transcripts on epithelial–mesenchymal transition (EMT).

Results : MicroRNA microarray profiling revealed hsa-miR-148a-3p was the most redundantly detected transcript in SRF and vitreous fluid from patients with RD, but not those with the other diseases. Expression levels of hsa-miR-148a-3p were higher in SRF samples than in blood serum samples in 3 out of 4 patients. Following hsa-miR-148a-3p mimic transfection, ARPE-19 and human RPE cells demonstrated increased expression of alpha-smooth muscle actin by western blotting and increased migration ability during scratch assays.

Conclusions : The results of the present study indicate hsa-miR-148a-3p was specifically detected in RD and promotes EMT in RPE.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×