September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
The importance of Caveolin-1 in the eyes with proliferative vitreoretinopathy
Author Affiliations & Notes
  • Yosuke Nagasaka
    Ohthalmology, Nagoya Univercity Graduate school of Medicine, Nagoya, Japan
    Ohthalmology, Tajimi Prefectural Hospital, Tajimi, Japan
  • Hiroki Kaneko
    Ohthalmology, Nagoya Univercity Graduate school of Medicine, Nagoya, Japan
  • Fuxiang Ye
    Ohthalmology, Nagoya Univercity Graduate school of Medicine, Nagoya, Japan
  • Kei Takayama
    Ohthalmology, Nagoya Univercity Graduate school of Medicine, Nagoya, Japan
  • Hiroko Terasaki
    Ohthalmology, Nagoya Univercity Graduate school of Medicine, Nagoya, Japan
  • Footnotes
    Commercial Relationships   Yosuke Nagasaka, None; Hiroki Kaneko, None; Fuxiang Ye, None; Kei Takayama, None; Hiroko Terasaki, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5366. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yosuke Nagasaka, Hiroki Kaneko, Fuxiang Ye, Kei Takayama, Hiroko Terasaki; The importance of Caveolin-1 in the eyes with proliferative vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5366.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Previous studies showed strong involvement of epithelial-mesenchymal transition (EMT) of the retinal pigment epithelial (RPE) cells in the pathogenesis of proliferative vitreoretinopathy (PVR). On the other hand, Caveolin-1, principal protein component of caveolae, has been reported to play an important role in EMT. In this study, we investigated the involvement of Caveolin-1 in EMT and PVR pathogenesis, using tissue samples from PVR eyes, mice with surgically induced PVR and different cell line of RPE.

Methods : Fibro-vascular membrans excised from 3 eyes with PVR were immunostained with anti-Caveolin-1 antibody. C57BL6J and Caveolin-1 knock out (Cav-1-/-) mice were obtained, and PVR was induced surgically. The retina/RPE lysates obtained from PVR eyes were analyzed with Western Blot (WB). Primary human RPE (hRPE) cells and ARPE-19 cells transfected with siRNA_CAV-1, and primary mouse RPE cells collected from C57BL6J and Cav-1-/- mice were analyzed by WB with anti-αSMA antibody and scratch assay.

Results : All tissue samples from PVR eyes were specifically stained with Caveolin-1. Retina/RPE samples from PVR eyes with Cav-1-/- mice strongly expressed αSMA compared to those with C57BL6J mice. hRPE with siRNA_ CAV-1 and primary mouse RPE cells from Cav-1-/- mice showed larger number of migrating cells in the scratch assay.

Conclusions : Caveolin-1 is expressed in the PVR tissue and prevents PVR by negatively regulating EMT in RPE cells.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×