September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Vitreous and subretinal fluid ATP concentrations in rhegmatogenous retinal detachment
Author Affiliations & Notes
  • Takashi Tachibana
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Toshio Hisatomi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shoji Notomi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shunji Nakatake
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yusuke Murakami
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Akihito Sengoku
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yasuhiro Ikeda
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shigeo Yoshida
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Koh-Hei Sonoda
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships   Takashi Tachibana, None; Toshio Hisatomi, None; Shoji Notomi, None; Shunji Nakatake, None; Yusuke Murakami, None; Akihito Sengoku, None; Yasuhiro Ikeda, None; Shigeo Yoshida, None; Tatsuro Ishibashi, None; Koh-Hei Sonoda, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5376. doi:
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      Takashi Tachibana, Toshio Hisatomi, Shoji Notomi, Shunji Nakatake, Yusuke Murakami, Akihito Sengoku, Yasuhiro Ikeda, Shigeo Yoshida, Tatsuro Ishibashi, Koh-Hei Sonoda; Vitreous and subretinal fluid ATP concentrations in rhegmatogenous retinal detachment. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5376.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Adenosine triphosphate (ATP) plays an important role in cell energy metabolism. During the course of apoptosis, dying cells release ATP into extracellular space. We previously reported that extracellular ATP accelerates photoreceptor cell apoptosis in subretinal hemorrhage. However, role of ATP in vitreous is not fully understood. The purpose of this study is to determine the vitreous levels of ATP and its correlation with clinical data in rhegmatogenous retinal detachment (RRD).

Methods : Vitreous samples were obtained from 58 eyes of 58 patients with RRD (n = 41), macular hole (MH; n=13), and epiretinal membrane (ERM; n =14) during pars plana vitrectomy. In RRD patient, subretinal fluids were also corrected from retinal tear during vitrectomy. We measured ATP concentration using luciferin-luciferase assay.

Results : Compared with MH and ERM patient, RRD patient have higher vitreous ATP concentration (RRD: 1.74ng/ml, MH: 0.11ng/ml, ERM: 0.065ng/ml, p < 0.005, respectively). In RRD patient, subretinal fluid contains higher ATP than vitreous fluid (vitreous fluid: 1.74ng/ml, subretinal fluid: 13.72ng/ml, p<0.005).

Conclusions : RRD patient have high ATP concentration in vitreous. High ATP concentration in vitreous and subretinal fluid of RRD may be associated with photoreceptor apoptotic cell death.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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