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Xun Xu, Shaopin Zhu, Xiaolu Yang; Inhibition of corneal inflammation by a small peptide derived from human pancreatitis-associated protein. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5407.
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© ARVO (1962-2015); The Authors (2016-present)
JY-8 is a novel 16-amino-acid peptide indentified from human pancreatitis-associated protein (PAP), a protein with protective effect against inflammatory diseases. In this study, we investigated the effect of JY-8 on poly(I:C) and LPS induced keratitis using in vitro and in vivo assays.
ELISA and RT-PCR were used to test the expression of cytokines; Clinical scoring and histological study were used to evaluate the inflammatory reaction of the cornea in vivo; The expression of ICAM-1 and phosphorylation of IKKα/β / IκBα / NF-κB was detected by immunofluorescence and immunoblot analyses.
Our results suggested that JY-8 suppressed the poly(I:C) and LPS-induced mRNA and protein expression of interleukin (IL)-1beta, IL-6, IL-8 and monocyte chemotactic protein (MCP)-1 in the corneal fibroblasts in vitro(P < 0.01 vs. the PBS group), inhibited the clinical manifestation, neutrophil infiltration, and expression of inflammatory cytokines in the cornea. We also demonstrated the possible mechanism of JY-8 on keratitis was through interrupting the NF-κB pathway.
JY-8, a novel peptide derived from human protein, may be a promising and safe drug for therapeutic application for corneal inflammation.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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