September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Inhibition of uveitis by intravitreal injection of a small peptide derived from hepatocyte growth factor
Author Affiliations & Notes
  • Weijun Wang
    Ophthalmology, Shang Hai First People's Hospital, Shanghai, China
  • Lu Cheng
    Ophthalmology, Shang Hai First People's Hospital, Shanghai, China
  • Shaopin Zhu
    Ophthalmology, Shang Hai First People's Hospital, Shanghai, China
  • Xun Xu
    Ophthalmology, Shang Hai First People's Hospital, Shanghai, China
  • Footnotes
    Commercial Relationships   Weijun Wang, None; Lu Cheng, None; Shaopin Zhu, None; Xun Xu, None
  • Footnotes
    Support  National Natural Science Foundation of China (81273424)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5413. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Weijun Wang, Lu Cheng, Shaopin Zhu, Xun Xu; Inhibition of uveitis by intravitreal injection of a small peptide derived from hepatocyte growth factor. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5413.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : H-K120 is a novel 20-amino-acid peptide indentified from the hepatocyte growth factor, a protein with protective effect against inflammatory diseases. In this study, we investigated the anti-inflammation effect of H-K120 in models of experimental ocular inflammation, icluding endotoxin induced uveitis (EIU) and experimental autoimmune uveitis (EAU), using in vitro and in vivo assays.

Methods : ELISA and RT-PCR were used to test the expression of cytokines; Clinical scoring and histological study were used to evaluate the inflammatory reaction of EIU and EAU in vivo; The expression of ICAM-1, VCAM-1, E-selectin, and phosphorylation of IKKα/β / IκBα / NF-κB was detected by immunofluorescence and immunoblot analyses.

Results : Our results suggested that Our results suggested that intravitreal treatment of H-K120 concentration-dependently inhibited clinical manifestation, neutrophil infiltration and improved histopathologic scores in vivo. Moreover, H-K120 attenuated the LPS-induced mRNA and protein expression of interleukin (IL)-1beta, IL-6, IL-8 and tumor necrosis factor (TNF)-a in RAW 264.7 cells and inhibited cell chemotactic migration toward LPS. We also demonstrated that H-RN suppressed expression of adhesion molecule including ICAM-1, VCAM-1 and E-selectin in HUVECs. We also found the possible mechanism of H-K120 on uveitis was through interrupting the NF-κB pathway.

Conclusions : H-K120, a novel peptide derived from hepatocyte growth factor, may be a promising and safe drug for therapeutic application for corneal inflammation.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×