September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Individual components of a nutraceutical formula synergistically contribute to the effect on COX-2 and iNos activity and expression
Author Affiliations & Notes
  • Maria Grazia Mazzone
    Business and Portfolio Development, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A., Lavinaio - Aci Sant'Antonio, CT, Italy
  • Cristina Zappulla
    Research and Development, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A., Lavinaio - Aci Sant'Antonio , CT, Italy
  • Luca Rosario La Rosa
    Research and Development, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A., Lavinaio - Aci Sant'Antonio , CT, Italy
  • Manuela Santonocito
    Research and Development, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A., Lavinaio - Aci Sant'Antonio , CT, Italy
  • Santa Viola
    Research and Development, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A., Lavinaio - Aci Sant'Antonio , CT, Italy
  • Francesco Giuliano
    Research and Development, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A., Lavinaio - Aci Sant'Antonio , CT, Italy
  • Footnotes
    Commercial Relationships   Maria Grazia Mazzone, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A. (E); Cristina Zappulla, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A. (E); Luca La Rosa, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A. (E); Manuela Santonocito, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A. (E); Santa Viola, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A. (E); Francesco Giuliano, S.I.F.I. (Società Industria Farmaceutica Italiana) S.p.A. (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5417. doi:
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      Maria Grazia Mazzone, Cristina Zappulla, Luca Rosario La Rosa, Manuela Santonocito, Santa Viola, Francesco Giuliano; Individual components of a nutraceutical formula synergistically contribute to the effect on COX-2 and iNos activity and expression. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5417.

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      © ARVO (1962-2015); The Authors (2016-present)

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  • Supplements
Abstract

Purpose : Anti-VEGF agents currently available for the treatment of Diabetic Macular Edema and Wet Age-Related Macular Degeneration do not possess a direct effect on inflammation. Here we set out to investigate whether a nutritional supplement may aid the activity of anti-VEGF drugs by providing anti-inflammatory support.

Methods : A formula codenamed AVS (SIFI S.p.A.) (1.9 mg/ml) and its individual components (astaxanthin, piperine, boswellic acid, bromelain, coenzyme Q10, copper, curcumin, lutein, anthocyanin, resveratrol, safranal, salicin, zeaxanthin, zinc) were tested on J774.2 cells. In particular, sub-confluent cells were pretreated (2 h) with AVS or individual components. Following LPS (1 µg/ml) treatment and overnight incubation, the medium was collected and nitrites and PGE2 accumulation determined. Total RNA was also extracted to assess the expression of iNos and COX-2 mRNAs by Real Time RT-PCR. Data represent the average of 6 replicates from 3 different experiments. Statistical analysis were performed using one-way ANOVA plus Dunnett’s post hoc test.

Results : LPS induced the accumulation of ∼24 µmol/l nitrites and of ∼21 ng/ml PGE2. Treatment with AVS, bromelain, zinc, salicin or anthocyanin effectively inhibited the accumulation of nitrites by 63-90% (p≤0.0001). Similarly AVS, bromelain or anthocyanin significantly inhibited PGE2 accumulation by 38-99% (p≤0.05, p≤0.0001). The inhibition of nitrites and PGE2 accumulation was paralleled by a significant inhibition of iNos (∼95%) and COX-2 (60-85%) expression, respectively. Components inactive when tested individually showed a synergistic effect on inhibition of nitrites and PGE2 accumulation when mixed together (MixJ). This effect was paralleled by a significant inhibition of iNos expression (94%) while COX-2 inhibition (55%) failed to reach statistical significance.

Conclusions : The AVS formula was effective in inhibiting nitrites and PGE2 accumulation in J774.2 cells. These activities appear to rely upon inhibition of iNos and COX-2 expression. Interestingly, components found to be inactive when tested individually were found to act synergistically when tested as a mix. Therefore, the AVS nutritional formula appears to be endowed with anti-inflammatory properties that may well prove useful to support anti-VEGF therapy.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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