September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Choroidal and retinal thickness in normal and autonomic denervated eyes: preliminary OCT-data in adult chicken
Author Affiliations & Notes
  • Alexandra Kaser-Eichberger
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
  • Falk Schroedl
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
    Dept. of Anatomy, Paracelsus Medical University , Salzburg, Austria
  • Andrea Trost
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
  • Barbara Bogner
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
  • Christian Runge
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
  • Karolina Motloch
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
  • Daniela Bruckner
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
  • Frank Schaeffel
    Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Tübingen, Germany
  • Marita P Feldkaemper
    Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Tübingen, Germany
  • Herbert Reitsamer
    Ophthalmology and Optometry, Paracelsus Medical University / SALK, Salzburg, Austria
  • Footnotes
    Commercial Relationships   Alexandra Kaser-Eichberger, None; Falk Schroedl, None; Andrea Trost, None; Barbara Bogner, None; Christian Runge, None; Karolina Motloch, None; Daniela Bruckner, None; Frank Schaeffel, None; Marita Feldkaemper, None; Herbert Reitsamer, None
  • Footnotes
    Support  PMU/FFF grant E-13/17/088-KAS
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5519. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Alexandra Kaser-Eichberger, Falk Schroedl, Andrea Trost, Barbara Bogner, Christian Runge, Karolina Motloch, Daniela Bruckner, Frank Schaeffel, Marita P Feldkaemper, Herbert Reitsamer; Choroidal and retinal thickness in normal and autonomic denervated eyes: preliminary OCT-data in adult chicken. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5519.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : For small eyes, high frequency ultrasound is the method of choice to measure changes in the various layers of the chicken posterior eye. Here we ask if optical coherence tomography (OCT) represents an alternative method for the larger adult eye, and if these measurements are also feasible in awake animals. Further, we compare data of normal eyes with autonomic denervated eyes, in which sympathetic and parasympathetic innervation is surgically removed.

Methods : In 8 mature chickens (1200 gr body weight), transection of the two parasympathetic pathways (ciliary ganglion, pterygopalatine ganglion) and sympathetic pathway (superior cervical ganglion) was performed unilaterally. The contralateral unlesioned eye served as a control. Seven days post surgery, choroidal thickness was measured in alert, hand-held animals with OCT (Spectralis, Heidelberg Engineering). Choroidal and retinal thickness was measured at 3 to 5 spots on at least 3 OCT-scans per eye, and data compared using a paired Student`s t-test. Additionally, interocular pressure (IOP) was measured using a rebound tonometer.

Results : Repetitive OCT-measurements are feasible in the awake animals. Choroids in lesioned sites were significantly thinner compared to contralateral controls (177 ± 9 µm on the lesioned side vs. 207 ± 10 µm in the contralateral control; paired t-test, p = 0.04) while retinal thickness was unaffected (195 ± 6 µm on the lesioned side vs. 189 ± 5 µm on the contralateral control side; paired t-test, p = 0.33). IOP in the lesioned side at the end of the experiments (day 6 post surgery) was significantly higher (lesioned eye versus control eye: 14 ± 3 mm Hg versus 38 ± 5 mm, p = <0.001).

Conclusions : OCT-measurements are a valuable tool to detect thickness changes in posterior layers of adult and awake animals, and repetitive measurements under various experimental manipulations are possible, including off-axis measurements in future applications. The observed reduced IOP in lesioned sides might contribute to the observed choroidal thinning. If this is a consequence of reduced blood flow, reduced aqueous humor production or reduced filling of the choroidal lacunae remains to be elucidated.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×