September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Characterization of the Elastic Properties of Lower Field Myopia in Guinea Pig Eyes at the Micrometer Scale with Acoustic Microscopy
Author Affiliations & Notes
  • Quan V Hoang
    Department of Ophthalmology, Harkness Eye Institute, Columbia University , New York, New York, United States
  • Daniel Rohrbach
    F. L. Lizzi Center for Biomedical Engineering, Riverside Research, New York, New York, United States
  • Quan Wen
    Department of Ophthalmology, Harkness Eye Institute, Columbia University , New York, New York, United States
  • Sally A McFadden
    Vision Sciences Group, Faculty of Science and IT, School of Psychology, University of Newcastle, Callaghan, New South Wales, Australia
  • Ronald H Silverman
    Department of Ophthalmology, Harkness Eye Institute, Columbia University , New York, New York, United States
    F. L. Lizzi Center for Biomedical Engineering, Riverside Research, New York, New York, United States
  • Jonathan Mamou
    F. L. Lizzi Center for Biomedical Engineering, Riverside Research, New York, New York, United States
  • Footnotes
    Commercial Relationships   Quan Hoang, None; Daniel Rohrbach, None; Quan Wen, None; Sally McFadden, None; Ronald Silverman, None; Jonathan Mamou, None
  • Footnotes
    Support  This work was supported in part by an unrestricted grant from Research to Prevent Blindness (RPB), NIH Core Grant P30EY019007, NIH Grant R21EB016117 (JM), Hunter Medical Research Institute HMRI G1400967 (SAM) and Career Development Awards from Research to Prevent Blindness (QVH), K08 Grant (QVH, 1 K08 EY023595, National Eye Institute, NIH) and the Louis V. Gerstner Jr. Scholars Program (QVH) and philanthropic support from Joseph Connors (QVH).
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5532. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Quan V Hoang, Daniel Rohrbach, Quan Wen, Sally A McFadden, Ronald H Silverman, Jonathan Mamou; Characterization of the Elastic Properties of Lower Field Myopia in Guinea Pig Eyes at the Micrometer Scale with Acoustic Microscopy. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5532.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Innate regional differences in refractive error have been previously reported in guinea pig eyes in which the inferior visual field (viewing the ground) is more myopic than the superior visual field (viewing the upper horizon) (Zeng et al., 2012). During the development of visually-induced myopia, the sclera thins and remodels, potentially altering its elastic properties. In this study, we used scanning acoustic microscopy (SAM) to determine if regional differences also occur in the natural elastic properties of the superior and inferior sclera in young guinea pig eyes.

Methods : Five eyes from 14-day-old guinea pigs were enucleated and embedded in Tissue-Tek O.C.T. compound, flash-frozen, and vertically cryosectioned into 12-μm sections (n = 12) through either the midline of the optic nerve head, the nasal edge of optic nerve or the central axis of the eye. The SAM system used a F-1.16, 250-MHz transducer which yielded a 7-μm lateral focal-point beam width. To form 2D images, samples were raster scanned in 2D in 2-µm steps using high-precision motor stages and the amplified echo signals were processed to yield values for bulk modulus (K) and mass density (rho) at each location.

Results : The bulk modulus of the guinea pig posterior sclera varied depending on location, with K values in the inferior mid-peripheral sclera (2.7 ± 0.27 GPa) significantly greater than in the superior mid-peripheral sclera (2.65 ± 0.3 GPa, p < 0.001). The mass density also varied, with rho in the inferior mid-peripheral sclera (0.99 ± 0.06 g/cm3) significantly lower than that in the superior mid-peripheral sclera (1.01 ± 0.06 g/cm3, p < 0.001).

Conclusions : We demonstrate with fine-resolution, regional differences in the elasticity of the sclera. These differences are consistent with greater scleral rigidity in the inferior sclera, while the sclera in the myopic zone has relatively greater elasticity. It will be of future interest to determine if differences in scleral elasticity emerge during the development of visually-induced myopia.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×