September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Scleral Remodeling in Early Recovery from Induced Experimental Myopia
Author Affiliations & Notes
  • Denise Hileeto
    School of Optometry and Vision Science, University of Waterloo, Waterloo, Ontario, Canada
  • Elizabeth L Irving
    School of Optometry and Vision Science, University of Waterloo, Waterloo, Ontario, Canada
  • Footnotes
    Commercial Relationships   Denise Hileeto, None; Elizabeth Irving, None
  • Footnotes
    Support  NSERC
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5534. doi:
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      Denise Hileeto, Elizabeth L Irving; Scleral Remodeling in Early Recovery from Induced Experimental Myopia. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5534.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigated the contribution and impact of fibrous scleral remodeling in the early recovery from lens induced myopia.

Methods : Myopia was induced by applying -10D goggles unilaterally to chicks on the day of hatching. Chicks were raised in a 14/10 hour light/dark cycle. Food and water were provided ad libitum. Experimental animals were assigned randomly to 3 groups – myopia (n=6), myopia with early recovery (3 hours post goggle removal, n=7), and normal control (n=6). After 14 days, eyes were enucleated immediately after euthanasia and fixed[IE1] for histopathologic evaluation. Consecutive sections of the entire ocular globe were processed for microscopic analysis. The histological slides were evaluated with bright field microscopy using Leica image analysis software for thickness of the fibrous sclera, fibroblast density, fibroblast morphometric parameters, and elastic fiber contents.

Results : Early recovery from myopia resulted in significant increase in the thickness of fibrous sclera to levels twice as high as the ones observed in normal controls or induced myopia (p<0.001). There was marked increase in fibroblast proliferation in the early myopia recovery group compared to myopia and normal control groups, evident by significantly increased cell densities (reflected in fibroblast counts per standard area, p< 0.05). There was no difference in the elastic fiber contents between the normal and myopic group, however the process of early recovery was associated with a statistically significant increase in the amount of elastic fibers in the early recovery group. Fibroblast morphometry revealed no difference between any of the experimental groups in terms of average cell dimensions including maximum cell diameter, perimeter, and area.

Conclusions : Recovery from induced experimental myopia causes fibrous scleral remodeling. The scleral contribution to early myopia recovery involves significant increase in the wall thickness, related to increased cell proliferation and increased elastic fiber contents. These results indicate that the fibrous sclera is not a passive component in the emmetropization process, but rather plays a significant active role in the adaptation changes in the eye in early recovery from induced myopia.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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