September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Effects of Nicotine on the ERG b-Wave Amplitude of the Superfused Isolated Mammals Retina
Author Affiliations & Notes
  • Siarhei Siapich
    RWTH Aachen University, Aachen, Germany
    Eye Clinic Dardenne, Bonn, Germany
  • Janina Mauer
    RWTH Aachen University, Aachen, Germany
  • Peter Walter
    RWTH Aachen University, Aachen, Germany
  • Footnotes
    Commercial Relationships   Siarhei Siapich, None; Janina Mauer, None; Peter Walter, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5770. doi:
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      Siarhei Siapich, Janina Mauer, Peter Walter; Effects of Nicotine on the ERG b-Wave Amplitude of the Superfused Isolated Mammals Retina. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5770.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Nicotine is a substance, that is taken very often world wide, aktive or passive, or on purpose of therapy. Nicotine acetylcholine receptors were found on the retinal amacrine cells, contacted to bipolars. The functional changes caused by nicotine application are of particular interest in our research. In this study we present the effects of nicotine on the retinal signal transduction, with a focus on the bipolar retinal cells of the inner nuclear layer, generating b-wave response of electroretinogram (ERG) of isolated superfused bovine retinas.

Methods : We have used a superfused vertebrate retina assay, testing retina from bovine. The retina was separated from the underlying pigment epithelium and mounted on a mesh placed in the centre of the perfusing chamber. The electroretinogram was recorded in the surrounding nutrient oxygenated phosphate buffered saline (PBS) via two Ag/AgCl-electrodes on either side of the retina. The recording chamber containing a piece of retina was placed in an electrically and optically insulated Faraday's cage. The retina was dark-adapted and the ERG was elicited at intervals of five minutes using a single light flash (10 mlux) for stimulation. After reaching stable ERG amplitudes, nicotine (2 µM, 5 µM und 20 µM) was added to the perfusing solution. After 60 min the nicotine was washed out for one hour by perfusion with PBS. Changes in b-wave amplitude before, during and after nicotine application were calculated and plotted.

Results : 2µM nicotine increased the b-wave amplitude to 1.6 fold in average (n=3). 5µM nicotine had even more pronounced stimulatory effect, increasing the b-wave amplitude from 2.8 up to 4.9 fold (n=3). At both concentrations the effect was reversible after PBS wash-out. 20µM nicotine showed a clear toxic effect on the b-wave amplitude, showing a 3.5 fold decrease of b-wave amplitude with almost no recovery after wash out (n=3).

Conclusions : The stimulation of the b-wave amplitude at 2 and 5 µM nicotine concentrations could be caused by enhanced signal transduction with involvement of collateral retinal pathways in dark adapted retina, probably involving amacrine cells, thus having influence on the nocturnal vision. At 20µM nicotine concentration the retinal toxicity is observed, with poor functional recovery after the drug is removed.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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