September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
PDGF-BB enhances the proliferation of cells in human orbital fibroblasts by suppressing PDCD4 expression via the up-regulation of microRNA-21
Author Affiliations & Notes
  • Ji-Sun Paik
    Ophthalmology and Visual Science, The Catholic University of Korea, Seoul St. Mary's Hospital , Seoul, Seoul, Korea (the Republic of)
  • Seong-Beom Lee
    Pathology, The Catholic University of Korea, Seoul, Korea (the Republic of)
  • Suk-Woo Yang
    Ophthalmology and Visual Science, The Catholic University of Korea, Seoul St. Mary's Hospital , Seoul, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Ji-Sun Paik, None; Seong-Beom Lee, None; Suk-Woo Yang, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5780. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Ji-Sun Paik, Seong-Beom Lee, Suk-Woo Yang; PDGF-BB enhances the proliferation of cells in human orbital fibroblasts by suppressing PDCD4 expression via the up-regulation of microRNA-21. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5780.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The aim of this study was to investigate the effect of platelet-derived growth factor (PDGF)-BB on the proliferation of cells and its possible mechanism in human orbital fibroblasts.

Methods : Human orbital fibroblasts were obtained from orbital fat from decompression surgery in patients with thyroid-associated ophthalmopathy (TAO). The cells were treated with PDGF-BB and the number of cells number was counted using an ADAM automatic cell counter. The expression of Programmed cell death 4 (PDCD4) was determined by western blotting. The effect of PDCD4 on cell proliferation was evaluated using PDCD4 siRNA treansfected cells. The level of microRNA (miRNA)-21 was measured by quantitative real-time RT-PCR. In addition, the role of miRNA-21 in PDCD4 expression and the proliferation of PDGF-BB-treated cells was assessed using anti-miRNA-21 transfected cells and by treatment with resveratrol, an inhibitor of miRNA-21.

Results : PDGF-BB was found to enhance cell proliferation, whereas it inhibited PDCD4 expression in human orbital fibroblasts. The down-regulation of PDCD4 by PDCD4 siRNA transfection significantly increased the number of human orbital fibroblasts. In addition, PDGF-BB increased the level of miRNA-21 in human orbital fibroblasts. Transfection with anti-miRNA-21 and treatment with resveratrol partially restored the expression of PDCD4 and led to a reduction in cell number in PDGF-BB treated orbital fibroblasts.

Conclusions : PDGF-BB enhances proliferation by suppressing PDCD4 expression via the up-regulation of miRNA-21 in human orbital fibroblasts. These results suggest that PDGF-BB stimulates cell proliferation through microRNA-21-mediated PDCD4 down-regulation, subsequently leading to the development of TAO.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×