September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Retinal enrichment in omega-3 polyunsaturated fatty acids limits choroidal neovascularization
Author Affiliations & Notes
  • Niyazi Acar
    Eye and Nutrition Research Group, INRA, CNRS, University of Burgundy, Dijon, France
  • Nina Kirmann
    Lipids, Nutrition and Cancer, UMR INSERM U866, Dijon, France
  • Julie Mazzocco
    Eye and Nutrition Research Group, INRA, CNRS, University of Burgundy, Dijon, France
  • Sandrine Bellenger
    Lipids, Nutrition and Cancer, UMR INSERM U866, Dijon, France
  • Stephane Gregoire
    Eye and Nutrition Research Group, INRA, CNRS, University of Burgundy, Dijon, France
  • Michel Narce
    Lipids, Nutrition and Cancer, UMR INSERM U866, Dijon, France
  • Lionel Bretillon
    Eye and Nutrition Research Group, INRA, CNRS, University of Burgundy, Dijon, France
  • Jerome Bellenger
    Lipids, Nutrition and Cancer, UMR INSERM U866, Dijon, France
  • Footnotes
    Commercial Relationships   Niyazi Acar, None; Nina Kirmann, None; Julie Mazzocco, None; Sandrine Bellenger, None; Stephane Gregoire, None; Michel Narce, None; Lionel Bretillon, None; Jerome Bellenger, None
  • Footnotes
    Support  National Institute for Research on Agronomy (INRA); Regional Council of Burgundy; European Regional Development Fund (FEDER); LabEX LipSTIC (ANR-11-LABX-0021)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 5806. doi:
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      Niyazi Acar, Nina Kirmann, Julie Mazzocco, Sandrine Bellenger, Stephane Gregoire, Michel Narce, Lionel Bretillon, Jerome Bellenger; Retinal enrichment in omega-3 polyunsaturated fatty acids limits choroidal neovascularization. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5806.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Age-related Macular Degeneration (AMD) is the primary cause of blindness in elderly population of western countries. Prospective epidemiologic studies have reported that omega-3 polyunsaturated fatty acids (PUFAs) reduce the risk of progression to advanced AMD, and particularly to the neovascular forms. In this study, we have investigated the beneficial effects of omega-3 PUFA on choroidal neovascularization (CNV) in mice.

Methods : Fat-1 mice, endogenously converting omega-6 to omega-3 PUFA, and littermate controls were used at 4 months of age. CNV was induced unilaterally in eye fundus by photocoagulation using a 532-nm laser. Confocal scanning laser ophthalmoscopy was used to evaluate CNV development after 9, 11 and 16 days post-laser. CNV area was quantified at the levels of the choroid and the retina by indocyanin green angiography (ICGA) and fluorescein angiography (FLA), respectively. Retinas were isolated in order to evaluate the fatty acid content by gas chromatography and the expression of genes involved in angiogenesis by RT-qPCR (Vegfa, Vegfr1, Vegfr2, Angpt1, Angpt2, Tgf-b, Tie2, Serpin1).

Results : The retinal content in omega-3 PUFA was significantly higher in fat-1 mice compared to controls whereas that of omega-6 PUFA was lower. While the levels of DHA were unchanged, those of n-3 docosapentaenoic acid (DPAn-3) and eicosapentaenoic acid (EPA) showed a 2-fold and 17-fold increase, respectively. FLA did not reveal any difference between fat-1 and controls. ICGA showed a reduction of CNV of 38% (p=0.035) and of 42% (p=0.009) in fat-1 mice when compared to controls 11 and 16 days after laser treatment, respectively. The expression of genes involved in angiogenesis, including Vegfa, Vegfr2 and Angpt2, was significantly lowered in the laser-treated retinas of fat-1 mice compared to controls.

Conclusions : These results suggest that enriching the retina with omega-3 PUFAs may protect against the development of pathological angiogenesis. Our data are in accordance with human prospective studies suggesting that a higher dietary consumption of omega-3 PUFA reduce the risk for developing neovascular AMD.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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