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Ji-Ae Ko, Wakana Iwata, Chinami Hiraoka, Hideaki Okumichi, Yoshiaki Kiuchi; Efffcts of retino-collicular coculture system on the neuroprotection of retinal ganglion cells. Invest. Ophthalmol. Vis. Sci. 2016;57(12):6003.
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© ARVO (1962-2015); The Authors (2016-present)
The neuroprotection of retinal ganglion cells is important for development of new drugs for glaucoma. We have now examined the effects of midbrain on the retinal ganglion cells survival with coculture system.
Midbrain slices from 3days rats after birth and retina explantation, or primary retinal ganglion cells from rats were cocultured using 3D-transwell culture system. Using RT-PCR and immunoblot analysis, the expression levels of the several survival markers of retinal ganglion cells were studied. Also, the extension of neurites of retinal ganglion cells after coculture was examined by Immunofluorescence analysis.
RT-PCR, immunoblot analysis and extension of neurites were not changed in retinal ganglion cells with midbrain slices or not, in normal conditions. But, in additional oxidative stress system, we found increase in the expression of neural markers and extension of neurites after coculture with midbrain slices compared to without that in retinal ganglion cells. So, the coculture with midbrain was blocked the damage from oxidative stress in retinal ganglion cells.
These results suggest that coculture of retinal ganglion cells and midbrain separated by transwell insert system provides an in vitro model for studies of the interaction between the signals and its targets in vivo. The some secreted factors from midbrain may play an important role in the regulation of retinal ganglion cells survival.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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