September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Analysis of corneal subbasal nerve plexus from wide-area mosaics in healthy subjects and in type 2 diabetics
Author Affiliations & Notes
  • Neil S Lagali
    Ophthalmology, Linkoping University, Linkoping, Sweden
  • Stephan Allgeier
    Karlsruhe Institute of Technology, Karlsruhe, Germany
  • Pedro Guimaraes
    Dept of Information Engineering, University of Padova, Padova, Italy
  • Reza A Badian
    Buskerud and Vestfold University College, Drammen, Norway
  • Alfredo Ruggeri
    Dept of Information Engineering, University of Padova, Padova, Italy
  • Bernd Koehler
    Karlsruhe Institute of Technology, Karlsruhe, Germany
  • Tor Paaske Utheim
    University of Oslo, Oslo, Norway
  • Beatrice Bourghardt Peebo
    Ophthalmology, Linkoping University, Linkoping, Sweden
  • Magnus Peterson
    University of Uppsala, Uppsala, Sweden
  • Lars Dahlin
    Lund University, Lund, Sweden
  • Olov Rolandsson
    Umeå University, Umeå, Sweden
  • Footnotes
    Commercial Relationships   Neil Lagali, None; Stephan Allgeier, None; Pedro Guimaraes, None; Reza A Badian, None; Alfredo Ruggeri, None; Bernd Koehler, None; Tor Utheim, None; Beatrice Bourghardt Peebo, None; Magnus Peterson, None; Lars Dahlin, None; Olov Rolandsson, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 6158. doi:
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      Neil S Lagali, Stephan Allgeier, Pedro Guimaraes, Reza A Badian, Alfredo Ruggeri, Bernd Koehler, Tor Paaske Utheim, Beatrice Bourghardt Peebo, Magnus Peterson, Lars Dahlin, Olov Rolandsson; Analysis of corneal subbasal nerve plexus from wide-area mosaics in healthy subjects and in type 2 diabetics. Invest. Ophthalmol. Vis. Sci. 2016;57(12):6158.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Very little is known about the subbasal nerve plexus architecture and density over a wide field of view in type 2 diabetes. We therefore performed a cross-sectional observational study to examine qualitative plexus changes and subbasal nerve density in type 2 diabetes relative to an age- and sex-matched healthy control group.

Methods : Laser-scanning in vivo confocal microscopy (IVCM) with the HRT3-RCM system was performed in 82 subjects bilaterally (39 diabetics and 43 controls). Wide-area mosaics were reconstructed by an automated algorithm and subbasal nerves in all mosaics were traced manually and by a fully automated tracing algorithm. Subbasal nerve density was compared between diabetics and healthy groups and according to diabetes duration.

Results : 163 mosaics from 82 subjects were successfully reconstructed, representing a mean enhancement factor of 37 single confocal fields of view and mean area of 6 mm2 of the central cornea. Patterns of innervation of the subbasal plexus could be studied qualitatively, and revealed regional variations in nerve density and tortuosity. Nerve density in the mosaic was highly correlated between manual and fully automated methods of nerve tracing (R2 = 0.88, P < 0.001), while the automated technique gave a 100-fold improvement in the speed of analysis. The mosaic density in diabetics was significantly reduced relative to healthy subjects (P = 0.018), and mosaic density declined with progression of type 2 diabetes (ANOVA P = 0.036; Pearson P = 0.007).

Conclusions : A progressive degradation of the subbasal nerve plexus over a wide area is apparent in type 2 diabetes and this study establishes values for nerve density over a large area of the central cornea.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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