September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Tear Secretion Change by Environmental Factor in Mice
Author Affiliations & Notes
  • Kokoro Sano
    Department of Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Tokyo, Japan
  • Motoko Kawashima
    Department of Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Tokyo, Japan
  • toshihiro imada
    Department of Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Tokyo, Japan
  • Ryuji Hisamura
    Department of Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Tokyo, Japan
  • Shigeru Nakamura
    Department of Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Tokyo, Japan
  • Fumiya Izumiseki
    Department of Neuropsychiatry, Keio University, Tokyo, Japan
  • Kenji F Tanaka
    Department of Neuropsychiatry, Keio University, Tokyo, Japan
  • Mitsuhiro Watanabe
    Keio Research Institute at SFC, Keio University, Kanagawa, Japan
  • Masaru Mimura
    Department of Neuropsychiatry, Keio University, Tokyo, Japan
  • Kazuo Tsubota
    Department of Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Kokoro Sano, None; Motoko Kawashima, None; toshihiro imada, None; Ryuji Hisamura, None; Shigeru Nakamura, None; Fumiya Izumiseki, None; Kenji Tanaka, None; Mitsuhiro Watanabe, None; Masaru Mimura, None; Kazuo Tsubota, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 6182. doi:
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      Kokoro Sano, Motoko Kawashima, toshihiro imada, Ryuji Hisamura, Shigeru Nakamura, Fumiya Izumiseki, Kenji F Tanaka, Mitsuhiro Watanabe, Masaru Mimura, Kazuo Tsubota; Tear Secretion Change by Environmental Factor in Mice. Invest. Ophthalmol. Vis. Sci. 2016;57(12):6182.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : A number of people live in a high-stress environment and it has become a serious problem in modern society. Dry eye disease (DED) is a highly prevalent disease and its incidence is rapidly increasing. We investigated whether tear secretion is associated with stress by environmental factors or not, in mice experiments.

Methods :
Male C57BL/6JJcl mice were exposed to environment enrichment (EE, n=30) and compared to mice living in a standard environment (SE, n=28) for 4 weeks. EE is defined as a living environment with a combination of complex inanimate and social stimulation. It has been reported that brain derived neurotrophic factor (BDNF) gene expression in the hippocampus increases by EE. After 4 weeks, mice were restrained once as an acute stress test for 4 hours. Tear secretion was measured once a week, and pre- and post-acute stress test.. Next we used BDNF STOPtetO mice which were suppressed of BDNF gene expression. We divided wild type mice, hetero mice and homo mice into two groups, SE and EE respectively, and compared tear secretion among all groups.

Results : Tear secretion in the EE group was significantly higher (2.5±1.0 mm, 3.0±1.6mm, 4.2±1.3mm) than in the SE group (1.7±0.7 mm, 2.2±0.9mm, 2.8±1.2mm, P<0.01, P<0.05, P<0.01, respectively) at week 1, week 2 and week 3 in C57BL/6JJcl mice. After the acute stress test, tear secretion decreased only in the SE group (pre-: 3.8±1.9mm, post-: 2.3±1.7mm, P=0.06). Tear secretion in the EE group didn’t change after the acute stress. Tear secretion significantly decreased in BDNF STOPtetO hetero mice (1.9±1.0mm, P<0.05) and homo mice (0.5±0.5mm, P<0.01) compared to wild type mice (2.4±0.9mm). Tear secretion increased only in the EE group (4.0±1.6mm, P<0.01) compared to the SE group (2.4±1.1mm) in wild type mice at week 2. Tear secretion in hetero mice and homo mice didn’t change under EE compared to under SE.

Conclusions : The results of this study suggest that environmental factors are related to tear secretion. Moreover, these results suggest an association between tear secretion and BDNF gene expression.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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