September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Elevated intraocular pressure increases melatonin levels in the aqueous humour
Author Affiliations & Notes
  • Jesus J Pintor
    Dep. Bioquimica, F de Optica UCM, Madrid, Spain
  • Hanan Alkozi
    Dep. Bioquimica, F de Optica UCM, Madrid, Spain
  • Juan Sanchez-Naves
    Department of Ophthalmology, , Institut Balear Oftalmoligia, Palma de Mallorca, Spain
  • Maria Jesus Perez de Lara
    Dep. Bioquimica, F de Optica UCM, Madrid, Spain
  • Gonzalo Carracedo
    Dep. Optica II, F de Optica, Madrid, Spain
  • Begoña Fonseca
    Dep. Bioquimica, F de Optica UCM, Madrid, Spain
  • Alejandro Martinez Aguila
    Dep. Bioquimica, F de Optica UCM, Madrid, Spain
  • Footnotes
    Commercial Relationships   Jesus Pintor, None; Hanan Alkozi, None; Juan Sanchez-Naves, None; Maria Jesus Perez de Lara, None; Gonzalo Carracedo, None; Begoña Fonseca, None; Alejandro Martinez Aguila, None
  • Footnotes
    Support  SAF2013-44416-R, RETICS RD12/0034/0003 and Universidad Complutense PR1/07-14890
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 6401. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Jesus J Pintor, Hanan Alkozi, Juan Sanchez-Naves, Maria Jesus Perez de Lara, Gonzalo Carracedo, Begoña Fonseca, Alejandro Martinez Aguila; Elevated intraocular pressure increases melatonin levels in the aqueous humour. Invest. Ophthalmol. Vis. Sci. 2016;57(12):6401.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To study the levels of melatonin in the aqueous humour of normotensive and hypertensive IOP patients and to compare them in an animal model of glaucoma.

Methods : 37 eyes of 37 patients who underwent cataract surgery were included in the study and were divided into normotensive patients, with IOP below 21 mmHg (n=23) and hypertensive patients, with IOP > 21 mm Hg (n=14). Glaucomatous DBA/2J (n=6) and control C57BL/6J (n=6) mice presenting 3 and 15 months of age for each strain were also used. Human and mice aqueous humours were aspirated using a 30-gauge Rycrof cannula on a tuberculin syringe and further processed to quantify melatonin by HPLC analysis.

Results : Melatonin levels in normotensive patients (IOP below 21 mm Hg) presented values of 33.14 ± 11.61 ng/mL (n=23), while hypertensive patients (IOP above 21 mm Hg) showed melatonin concentrations of 95.87 ± 28.23 ng/mL (n=14) (p<0.039). Glaucoma mice presented melatonin values of 0.44 ± 0.06 ng/mL (at 3 months of age, before the pathology starts) which raised to 1.45 ± 0.18 ng/mL (at 15 months of age, when the pathology is fully established and IOP is maximum) (n= 6, p<0.001). Control mice did not significantly modified melatonin concentrations between 3 and 15 months of age.

Conclusions : Glaucoma patients with high IOP present increased concentrations of melatonin in their aqueous humour compared to normotensive patients. This has been confirmed in a glaucomatous animal model in which it has been possible to see a correlation between the development of the pathology, with an increase in IOP, and a concomitant elevation of melatonin in the aqueous humour.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×