September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
ROLE OF PROSTAGLANDINS AND ACETYLCHOLINE IN THE CONTRACTILE ACTIONS OF HYDROGEN SULFIDE-RELEASING COMPOUNDS IN THE BOVINE ISOLATED IRIS
Author Affiliations & Notes
  • Sunny E Ohia
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Evelyn Ajelabi
    Pharmacy Sciences, Creighton University, Omaha, Nebraska, United States
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Christina Anyikwa
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Christine Ly
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Cherie Chua
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Cheryl Chua
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Jenaye Robinson
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Leah Bush
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Catherine A Opere
    Pharmacy Sciences, Creighton University, Omaha, Nebraska, United States
  • Ya Fatou Njie-Mbye
    Pharmaceutical Sciences, Texas Southern University, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Sunny Ohia, None; Evelyn Ajelabi, None; Christina Anyikwa, None; Christine Ly, None; Cherie Chua, None; Cheryl Chua, None; Jenaye Robinson, None; Leah Bush, None; Catherine Opere, None; Ya Fatou Njie-Mbye, None
  • Footnotes
    Support  NIH/NEI Grant R15EY022215-01
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 6402. doi:
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    • Get Citation

      Sunny E Ohia, Evelyn Ajelabi, Christina Anyikwa, Christine Ly, Cherie Chua, Cheryl Chua, Jenaye Robinson, Leah Bush, Catherine A Opere, Ya Fatou Njie-Mbye; ROLE OF PROSTAGLANDINS AND ACETYLCHOLINE IN THE CONTRACTILE ACTIONS OF HYDROGEN SULFIDE-RELEASING COMPOUNDS IN THE BOVINE ISOLATED IRIS. Invest. Ophthalmol. Vis. Sci. 2016;57(12):6402.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Previously, we reported that hydrogen sulfide (H2S)-releasing compounds can relax pre-contracted porcine isolated irides, an effect that was dependent upon endogenous production of both H2S and prostaglandins, and on the activity of KATP channels (Ohia et al. Curr. Eye Res. 35: 402, 2010). In the present study, we investigate the pharmacological actions of H2S-releasing compounds (NaHS and L-cysteine) on basal tone in bovine isolated irides. Furthermore, we studied role of prostaglandins and acetylcholine in the responses elicited by the H2S-releasing compounds on this tissue.

Methods : Isolated bovine iris muscle strips were set up in an organ baths containing oxygenated Krebs buffer solution maintained at 37°C and gassed with 95% O2: 5% CO2. The muscle strips were set to a resting tension of 0.3 g and longitudinal isometric tension was recorded via a Grass FT03 force-displacement transducer and analyzed using PolyView Computer Software. Contractile responses were elicited by the H2S releasing compounds in the absence and presence cyclooxygenase (COX) inhibitors (flurbiprofen and indomethacin) or the muscarinic receptor blocker, atropine.

Results :
NaHS (1 nM – 10 µM) and L-cysteine (100 nM - 1 mM) caused a concentration-dependent contraction of isolated bovine irides yielding EC50 values of 10 nM and 30 µM, respectively. Both COX inhibitors, flurbiprofen (10 µM) and indomethacin (10 µM) abolished the contractile actions of NaHS and L-cysteine on this tissue. Likewise, atropine (1 nM – 10 nM) significantly (p < 0.001) antagonized the contractile response to NaHS and L-cysteine.

Conclusions : We conclude that H2S-releasing compounds can elicit contraction of isolated bovine irides, an effect that is dependent upon the production of endogenous prostaglandins and the release of acetylcholine.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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