September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Functional renin receptors in human Schlemm’s Canal cells
Author Affiliations & Notes
  • Jayter Silva Paula
    Department of Ophthalmology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
    Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina, United States
  • Kristin Perkumas
    Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina, United States
  • Nicole E Ashpole
    Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina, United States
  • W Daniel Stamer
    Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina, United States
  • Footnotes
    Commercial Relationships   Jayter Paula, None; Kristin Perkumas, None; Nicole Ashpole, None; W Stamer, None
  • Footnotes
    Support  São Paulo Research Foundation (FAPESP # 2014/26163-6)
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 6417. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Jayter Silva Paula, Kristin Perkumas, Nicole E Ashpole, W Daniel Stamer; Functional renin receptors in human Schlemm’s Canal cells
      . Invest. Ophthalmol. Vis. Sci. 2016;57(12):6417.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Intraocular pressure is set by resistance generation to aqueous humor outflow in the juxtacanicular region where trabecular meshwork (TM) and Schlemm’s canal (SC) cells interact. Elements of the renin-angiotensin system (RAS) may contribute to outflow resistance since modulation of some RAS enzymes decrease IOP in experimental glaucoma. Our objective is to examine the expression of the (pro)renin receptor (PRR) and evaluate the effects of renin on signaling in and protein expression by cultured human SC cells.

Methods : RNA isolated from two human SC and three TM cells strains at confluence was tested using RT-PCR for expression of renin and PRR. In parallel, one SC cell strain was treated with renin (10-6, 10-7, and 10-8M) in 1% FBS-low glucose DMEM for 48 h and fibronectin content in conditioned media was analyzed by Western blot. Beta-catenin localization in cells was assessed by immunofluorescence microscopy.

Results : PRR, but not renin, was expressed in both SC and TM cells strains. Immunofluorescence analysis showed predominantly a cytoplasmatic and plasma membrane distribution of beta-catenin in untreated SC cells, while beta-catenin translocated to nucleus in 53% of renin-treated cells. In a biphasic dose-dependent fashion, renin increased fibronectin secretion from SC cells. Renin also appeared to cause morphological alterations that needs to be characterized further.

Conclusions : Cultured human SC cells appear not to express renin, but have a functional system for responding to renin in the aqueous humor. Its role in resistance generation needs to be explored in future studies.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×