June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The effect of crosslinked CMHA-S gel drops on in vivo corneal chemical burns
Author Affiliations & Notes
  • Cassie Sprague
    Ocular Trauma, United States Army Institute of Surgical Research, San Antonio, Texas, United States
  • Barbara M Wirostko
    EyeGate Pharmaceutical, Inc., Salt Lake City , Utah, United States
  • Brenda Mann
    EyeGate Pharmaceutical, Inc., Salt Lake City , Utah, United States
    SentrX Animal Care , Salt Lake City , Utah, United States
  • Jennifer McDaniel
    Ocular Trauma, United States Army Institute of Surgical Research, San Antonio, Texas, United States
  • Gina L Griffith
    Ocular Trauma, United States Army Institute of Surgical Research, San Antonio, Texas, United States
  • Footnotes
    Commercial Relationships   Cassie Sprague, None; Barbara Wirostko, EyeGate Pharmaceutical, Inc. (F), EyeGate Pharmaceutical, Inc. (I), EyeGate Pharmaceutical, Inc. (E), EyeGate Pharmaceutical, Inc. (P); Brenda Mann, EyeGate Pharmaceutical, Inc. (F), EyeGate Pharmaceutical, Inc. (I), EyeGate Pharmaceutical, Inc. (E), SentrX Animal Care (I), SentrX Animal Care (C), SentrX Animal Care (P); Jennifer McDaniel, None; Gina Griffith, None
  • Footnotes
    Support  This work was supported by the U.S. Army Medical Research and Materiel Command (MRMC) Clinical and Rehabilitative Medicine Research Program.
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 131. doi:
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      Cassie Sprague, Barbara M Wirostko, Brenda Mann, Jennifer McDaniel, Gina L Griffith; The effect of crosslinked CMHA-S gel drops on in vivo corneal chemical burns. Invest. Ophthalmol. Vis. Sci. 2017;58(8):131.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Hyaluronic acid, a ubiquitously expressed polysaccharide, is of great interest in the bioengineering and regenerative medicine communities for use as an off-the-shelf biomaterial. Topical cross-linked carboxymethylated hyaluronic acid (CMHA-S) has been shown to facilitate the repair and regeneration of corneal injuries and chemical burns. Our purpose was to test the hypothesis that CMHA-S gel drops can be utilized to facilitate re-epithelialization of the corneal epithelium in corneal chemical burn injuries.

Methods : Burns 5.5 mm in diameter were created on 20 anesthetized, male New Zealand White rabbits by placing a circular filter paper soaked in 1N NaOH onto the central cornea for 30 seconds. Wounds were immediately rinsed with sterile buffered saline and evaluated using fluorescein staining and white light imaging. Animals were randomly grouped (n=5 per group) for no treatment or treatment with phosphate buffered saline (PBS) drops as a control 4X daily or with CMHA-S gel drops (SentrX Animal Care Remend® Corneal Repair Gel) 4X or 2X daily for 14 days. An additional 5 animals were left unwounded and treated with CMHA-S drops 4X daily for 14 days as a control group. At days 1, 3, 7, 14, and 30 post chemical burn creation, eyes were evaluated by white light imaging and fluorescein staining. Corneal histology was performed using H&E and Masson’s Trichrome stain.

Results : CHMA-S drops were biocompatible and did not lead to any clinical or histological pathology in uninjured eyes when treated 4X daily. CMHA-S gel treatment at 2X and 4X daily facilitated the healing of the corneal chemical burns 3 and 7 days post wounding when compared to control and non-treated corneal chemical wounds. Corneal burns treated with CMHA-S gel drops 2X and 4X daily were completely healed on day 3 and showed smaller epithelial defects on day 7 when compared to controls. There were no significant differences in the overall size or quality of the corneal scar 30 days post wounding between the treatment and control groups.

Conclusions : CMHA-S gel drops are biocompatible and may be utilized clinically to facilitate corneal epithelial wound closure in corneal chemical burn injuries.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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