June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Potential role of topical bovine colostrum in remodeling corneal epithelial cells after an acute ocular alkali burn in mice
Author Affiliations & Notes
  • Laura Di Meglio
    Ophthalmology , The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine , Baltimore , Maryland, United States
  • Andreina Tarff
    Ophthalmology , The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine , Baltimore , Maryland, United States
  • Rebecca Yee
    Molecular Microbiology & Immunology , Johns Hopkins University , Baltimore , Maryland, United States
  • Praveena Gupta
    Ophthalmology , The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine , Baltimore , Maryland, United States
  • Anvesh Annadanam
    Ophthalmology , The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine , Baltimore , Maryland, United States
  • Marisol del Valle Cano
    Ophthalmology , The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine , Baltimore , Maryland, United States
  • William May
    Ophthalmology , The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine , Baltimore , Maryland, United States
  • Ashley Behrens
    Ophthalmology , The Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine , Baltimore , Maryland, United States
  • Footnotes
    Commercial Relationships   Laura Di Meglio, None; Andreina Tarff, None; Rebecca Yee, None; Praveena Gupta , None; Anvesh Annadanam, None; Marisol Cano, None; William May, None; Ashley Behrens, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 155. doi:
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      Laura Di Meglio, Andreina Tarff, Rebecca Yee, Praveena Gupta, Anvesh Annadanam, Marisol del Valle Cano, William May, Ashley Behrens; Potential role of topical bovine colostrum in remodeling corneal epithelial cells after an acute ocular alkali burn in mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):155.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Chemical ocular insults are among the most devastating ophthalmological emergencies. Corneal surface cell re-epithelization without scarring by biological fluids has been observed clinically. Our purpose was to evaluate bovine colostrum (BC) as a potential source to preserve the phenotype of a restored epithelium by means of an experimental mouse ocular burn model.

Methods : Twenty-four 5-18 week-old, female and male C57BL/6 mice were interchangeable-categorized into two gender and age-matched groups according to the topical treatment that was administered. After inducing a corneal alkali burn in all left eyes of both groups with 2 μl of 0.15 mol/l sodium hydroxide, both eyes of group 1 were treated with 5-μl eye-drop of whole BC 4 times per day, and both eyes of group 2 were treated with 5-μl eye-drop of isotonic saline solution (SS). Over a timeline of eight days after the induction of the alkali burn, corneal epithelial defects were photographed and measured on days two, four, six and eight. Ocular burn damage was assessed with a pre-established classification in clock hours of the limbus. On day eight, both eyes of each mouse were enucleated and assessed histopathologically. Mann-Whitney test was used for statistical analysis.

Results : Group 1 (BC) had a significantly improved fluorescein staining score for limbal involvement compared with group 2 (SS) at day 4 (p =0.0130), day 6 (p <0.001) and day 8 (p <0.001). The difference between groups 1 and 2 for limbal involvement post burns, at day 2 before any intervention, was not statistically significant (p > 0.99). The overall change (difference in slope) in fluorescein staining for limbal involvement between days 2 and 8 was -0.1669 (p = 0.0063).

Conclusions : Corneal epithelium repair is an elaborated multilevel process requiring several entities such grow factors and chemokines, collectively promoting extracellular matrix formation, cell migration, adherence and proliferation. Our results show the properties of BC, suggesting its pluripotent role in triggering corneal tissue reformation without scarring. Theses findings would provide novel therapies for all sight-threatening corneal diseases.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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