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tao guo, Li Guo, Yuan Deng, Iok-Hou Pang, Xianqun Fan; Elevated Levels of TGFβ2 and sFRP1 in Aqueous Humor of Glaucomatous Patients. Invest. Ophthalmol. Vis. Sci. 2017;58(8):207.
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© ARVO (1962-2015); The Authors (2016-present)
To assess levels of transforming growth factor β2 (TGFβ2) and secreted frizzled-related protein 1 (sFRP1) in the aqueous humor(AH) of eyes with different types of glaucoma.
AH samples were collected from 36 patients (36 eyes). Those samples, which were divided into six groups averagely, respectively came from eyes with primary open angle glaucoma (POAG), chronic angle closure glaucoma (CACG), primary angle closure suspects (PACS), acute angle-closure glaucoma (AACG) with high intraocular pressure (IOP), AACG with low IOP and cataract serving as controls. Patients undergoing cataract or glaucoma surgery without associated significant intraocular pathology were selected for this study. At the time of surgery, a small amount of aqueous was withdrawn. The concentration of TGFβ2 and sFRP1 were measured by enzyme-linked immunosorbent assay .
The mean concentration (± standard error) of TGFβ2 in the AH of eyes with POAG (457.18±122.79 pg/ml) was significantly higher than that in eyes with controls ( 292.83±80.45 pg/ml) (P=0.02). Levels of sFRP1 in AH of AACG with high IOP eyes(2.18±0.73 ng/ml) were found to be elevated when comparing to those of controls（1.37±0.18 ng/ml）(P=0.04). There was no significant change in concentration of TGFβ2 between eyes with controls and CACG （339.42±129.44 pg/ml）, PACS（313.79±151.39 pg/ml）, AACG with high IOP（278.96±166.88 pg/ml） ,AACG with low IOP（329.91±127.63 pg/ml）, respectively. The mean concentration of sFRP1 in the AH of eyes with POAG (1.43±0.66 ng/ml), CACG（1.51±0.59 ng/ml），PACS（1.75±0.56 ng/ml），AACG with low IOP（1.84±1.12 ng/ml），were not significantly different from that in eyes with controls. No correlation between TGFβ2 or sFRP1 levels and age, gender, previous treatment or type of surgery was found.
Our results indicate that elevated levels of TGFβ2 may play an important role in the pathogenesis of POAG, while that of sFRP1 may correlate with the progression of AACG.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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