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JOSE NAVARRO-PARTIDA, Carlos Daniel Diaz-Palomera, RAMSES ROSALES DIAZ, ABRIL BERNARDETTE MARTINEZ-RIZO, Adolfo Daniel Rodriguez-Carrizalez, ARTURO SANTOS; An increased expression of IL-17 and Foxp3 is observed in contrast to other Th genes in primary pterygium. Invest. Ophthalmol. Vis. Sci. 2017;58(8):209.
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© ARVO (1962-2015); The Authors (2016-present)
An increasing number of pro-inflammatory cytokines, angiogenic and fibrogenic growth factors and their receptors have been related to pterygium (Pt). T helper cells CD4+ (Th) are an important constituent on Pt tissues, however The effect of Th cytokines on Pt pathogenesis has been poorly studied. To date, only interleukin 4 (IL-4) from Th cells has been linked to pterygium recurrence. Therefore, this study was undertaken to evaluate the expression of Th cytokines and Th transcription factors in primary Pt.
Pterygia heads were collected from 28 eyes of 28 Mexican patients undergoing primary pterygium excision with conjunctival autografting. Conjunctival biopsies from 8 patients undergoing cataract surgery were used as control group. Gene expression of the Th citokines; interferon gamma (IFN-γ), IL-13, IL-17, IL-10, as well as the gene expression of the Th transcription factors; T-bet, GATA3, Foxp3 and RORγt were analyzed by real time RT-PCR using TaqMan assays. The 2-ΔΔCT method was used for data analysis.
The expression of T-bet, GATA3, RORγt, IFN-γ, IL-13 and IL-10 were downregulated in Pt. Interestingly, the lowest transcript expression for IL-13 and GATA3 were reached at maximum corneal invasion. The expressions of IL-17 and Foxp3 were increased up to 6 fold changes in Pt tissues (P< 0.0001), and occurred for the latter gene in a Pt size and Pt extension dependent manner.
The increased expression of IL-17 and Foxp3 in Pt specimens suggest the presence of IL-17+Foxp3+ T cells, a subset of Th population which suppress T cell proliferation and promotes tumor progression. Therefore, IL17+Foxp3+ T cells could be a therapeutic target to prevent pterygium growth.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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