June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
An increased expression of IL-17 and Foxp3 is observed in contrast to other Th genes in primary pterygium
Author Affiliations & Notes
  • JOSE NAVARRO-PARTIDA
    DIVISION DE BIOTECNOLOGIA Y SALUD. CAMPUS GUADALAJARA., TECNOLOGICO DE MONTERREY, ZAPOPAN, JALISCO, Mexico
  • Carlos Daniel Diaz-Palomera
    UNIDAD ACADEMICA DE MEDICINA , UNIVERSIDAD AUTONOMA DE NAYARIT , TEPIC, NAYARIT, Mexico
  • RAMSES ROSALES DIAZ
    UNIDAD ACADEMICA DE MEDICINA , UNIVERSIDAD AUTONOMA DE NAYARIT , TEPIC, NAYARIT, Mexico
  • ABRIL BERNARDETTE MARTINEZ-RIZO
    UNIDAD ACADEMICA DE MEDICINA , UNIVERSIDAD AUTONOMA DE NAYARIT , TEPIC, NAYARIT, Mexico
  • Adolfo Daniel Rodriguez-Carrizalez
    CENTRO UNIVERSITARIO DE CIENCIAS DE LA SALUD, UNIVERSIDAD DE GUADALAJARA, GUADALAJARA, JALISCO, Mexico
  • ARTURO SANTOS
    DIVISION DE BIOTECNOLOGIA Y SALUD. CAMPUS GUADALAJARA., TECNOLOGICO DE MONTERREY, ZAPOPAN, JALISCO, Mexico
  • Footnotes
    Commercial Relationships   JOSE NAVARRO-PARTIDA, None; Carlos Diaz-Palomera, None; RAMSES ROSALES DIAZ, None; ABRIL MARTINEZ-RIZO, None; Adolfo Rodriguez-Carrizalez, None; ARTURO SANTOS, None
  • Footnotes
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Investigative Ophthalmology & Visual Science June 2017, Vol.58, 209. doi:
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      JOSE NAVARRO-PARTIDA, Carlos Daniel Diaz-Palomera, RAMSES ROSALES DIAZ, ABRIL BERNARDETTE MARTINEZ-RIZO, Adolfo Daniel Rodriguez-Carrizalez, ARTURO SANTOS; An increased expression of IL-17 and Foxp3 is observed in contrast to other Th genes in primary pterygium. Invest. Ophthalmol. Vis. Sci. 2017;58(8):209.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : An increasing number of pro-inflammatory cytokines, angiogenic and fibrogenic growth factors and their receptors have been related to pterygium (Pt). T helper cells CD4+ (Th) are an important constituent on Pt tissues, however The effect of Th cytokines on Pt pathogenesis has been poorly studied. To date, only interleukin 4 (IL-4) from Th cells has been linked to pterygium recurrence. Therefore, this study was undertaken to evaluate the expression of Th cytokines and Th transcription factors in primary Pt.

Methods : Pterygia heads were collected from 28 eyes of 28 Mexican patients undergoing primary pterygium excision with conjunctival autografting. Conjunctival biopsies from 8 patients undergoing cataract surgery were used as control group. Gene expression of the Th citokines; interferon gamma (IFN-γ), IL-13, IL-17, IL-10, as well as the gene expression of the Th transcription factors; T-bet, GATA3, Foxp3 and RORγt were analyzed by real time RT-PCR using TaqMan assays. The 2-ΔΔCT method was used for data analysis.

Results : The expression of T-bet, GATA3, RORγt, IFN-γ, IL-13 and IL-10 were downregulated in Pt. Interestingly, the lowest transcript expression for IL-13 and GATA3 were reached at maximum corneal invasion. The expressions of IL-17 and Foxp3 were increased up to 6 fold changes in Pt tissues (P< 0.0001), and occurred for the latter gene in a Pt size and Pt extension dependent manner.

Conclusions : The increased expression of IL-17 and Foxp3 in Pt specimens suggest the presence of IL-17+Foxp3+ T cells, a subset of Th population which suppress T cell proliferation and promotes tumor progression. Therefore, IL17+Foxp3+ T cells could be a therapeutic target to prevent pterygium growth.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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