June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Progressive changes in RCS rat retina during OCT/FAF-based in vivo imaging and their correlation with histological and visual functional analysis
Author Affiliations & Notes
  • Juan Carlos Martinez
    Ophthalmology, USC Roski Eye Institute/University of Southern California, Los Angeles, California, United States
    USC Institute of Biomedical Therapeutics/University of Southern California, Los Angeles, California, United States
  • Laura Liu
    Ophthalmology, Chang Gung Memorial Hospital, Linkou Medical Center, Taoyuan, Taiwan
    Chang Gung University College of Medicine, Taoyuan, Taiwan
  • Juan Carlos Gutiérrez-Hernández
    Ophthalmology, Universidad de Costa Rica, San Jose, Costa Rica
    Hospital Clinica Biblica, San Jose, Costa Rica
  • Alejandra Gonzalez Calle
    USC Viterbi School of Engineering, University of Southern California, Los Angeles, California, United States
  • Biju Thomas
    Ophthalmology, USC Roski Eye Institute/University of Southern California, Los Angeles, California, United States
    USC Institute of Biomedical Therapeutics/University of Southern California, Los Angeles, California, United States
  • Mark S Humayun
    Ophthalmology, USC Roski Eye Institute/University of Southern California, Los Angeles, California, United States
    USC Institute of Biomedical Therapeutics/University of Southern California, Los Angeles, California, United States
  • Footnotes
    Commercial Relationships   Juan Carlos Martinez, None; Laura Liu, None; Juan Carlos Gutiérrez-Hernández, None; Alejandra Gonzalez Calle, None; Biju Thomas, None; Mark Humayun, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 281. doi:
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    • Get Citation

      Juan Carlos Martinez, Laura Liu, Juan Carlos Gutiérrez-Hernández, Alejandra Gonzalez Calle, Biju Thomas, Mark S Humayun; Progressive changes in RCS rat retina during OCT/FAF-based in vivo imaging and their correlation with histological and visual functional analysis. Invest. Ophthalmol. Vis. Sci. 2017;58(8):281.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine histological and visual functional correlates of changes observed during fundus autofluorescence (FAF) and optical coherence tomography (OCT) imaging of Royal College of Surgeon (RCS) rats during the progression of retinal degenerative (RD) disease.

Methods : FAF and retinal layer measurements were performed using Heidelberg Spectralis OCT at various postnatal ages (P18 to P180). Visual function was assessed using optokinetic nystagmus (OKN) testing. Retinal sections were stained with H&E, and retinal thickness (RT) and outer nuclear layer (ONL) cell count was analyzed using Aperio ScanScope. Morphological assessments and spectral analysis were used to ascertain correlation between progressive changes in the subretinal debris and the FAF pattern. Immunofluorescent staining for CD68 and GFAP (macrophage and glial cell markers) was performed to assess the source of hyperautofluorescence.

Results : FAF showed homogenous autofluorescence patterns up to P30, followed by homogeneous hyperautofluorescence. OCT showed severe loss of RT in young rats at P37, mostly due to loss of the ONL as demonstrated by histological analysis. Positive correlation between ONL thickness and OKN visual responses was observed at P30 and P37 (Pearson test, r=0.95). No OKN visual responses were recorded after P120. After P60, a hypoautofluorescence developed near the optic nerve. This area gradually spread by P90 and persisted through the entire study. ONL thickness loss and appearance of hypoautofluorescence were positively correlated (p<0.05). Bright hyperautofluorescent spots began appearing in a punctate fashion at P90. Morphological analysis suggested small patches of debris attached to the RPE surface, causing hyperautofluorescence and ruling out the contribution of macrophages and glial cells. Based on spectral analysis, hyperautofluorescent spots had a peak emission wavelength of 538 nm at P60 and P90, with higher wavelengths after P120. CD68 expression was found at P25 in the outer plexiform layer and ONL. By P46, CD68 was expressed predominantly in the outer segments.

Conclusions : Our study demonstrates that changes in FAF pattern can be predictive of morphological anomalies and visual functional loss in RCS rats. FAF is mostly due to subretinal debris as opposed to macrophages and other components within the RPE.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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