June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Expeditious bilateral murine fundus fluorescein angiography (FFA): Maximizing optical imagery with a transportable digital camera
Author Affiliations & Notes
  • Moshe Ehrenberg
    Institute of Drug Research, Hebrew University, Jerusalem, Israel, Israel
  • Scott Ehrenberg
    Technion Medical School, Haifa, Israel
  • Ouri Schwob
    Institute of Drug Research, Hebrew University, Jerusalem, Israel, Israel
  • Ofra Benny
    Institute of Drug Research, Hebrew University, Jerusalem, Israel, Israel
  • Footnotes
    Commercial Relationships   Moshe Ehrenberg, None; Scott Ehrenberg, None; Ouri Schwob, None; Ofra Benny, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 42. doi:
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      Moshe Ehrenberg, Scott Ehrenberg, Ouri Schwob, Ofra Benny; Expeditious bilateral murine fundus fluorescein angiography (FFA): Maximizing optical imagery with a transportable digital camera. Invest. Ophthalmol. Vis. Sci. 2017;58(8):42.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Experimental murine retinal angiography has become important as new drugs are being examined for treating macular degeneration. As laser is utilized to induce the experimental model of choroidal neovascularization (CNV) in mice, there has been an increasing demand for a compact fluorescein fundus camera. We have modified the use of the Kowa Genesis Df Camera to more efficiently implement bilateral Fundus Fluorescein Angiography (FFA) in rodents.

Methods : We operated this one kg camera that allows digital FFA recording to a Compact Flash memory card. In order to permit the appropriate focus and stability for photographing the delicate murine central fundus, the Indirect Lens Holder securely sets a 90 diopter lens at a fixed distance from the murine cornea and the camera's objective lens. In addition, one 12 mm circular micro coverslip is applied on each anesthetized cornea of the sedated mouse with Carbomer ophthalmic gel. Furthermore, a customized acrylic platform with acrylic rim is adapted to have the mouse comfortably maintain steady body, head and eye positions. Finally, a tripod, with a provisionary ball head and clamp, is used for controlled and precise flexibility of the camera's bearings.

Results : We were able to record robust in-vivo FFA images of the central murine fundus, of both eyes, during the arterio-venous, mid-venous, late-venous and late phases. The combination of the bilateral improvised contact lenses applied with an ophthalmic lubricant, the lightweight fluorescein camera, the specialized camera tripod and the customized acrylic platform and mandible rim, allowed us to efficaciously achieve bilateral rodent FFA without the inconveniences of corneal dehydration and secondary opacification, the generated fatigue and instability of operating a heavy camera, and the disruption of the experimental creature's equilibrium.

Conclusions : We were able to provide precise, reliable and bilateral FFA digital images of the central mouse fundus by adapting the use of a mobile recording device with secure refractive attachments, by sustained bilateral corneal lubrication, and by maximal stabilization of the murine body, head and ocular positions. As a result, we have been able to efficiently record the alterations in the central choroidal and retinal vasculature following laser-induced CNV in the experimental murine model of exudative macular degeneration.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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