June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Quantified elasticity mapping of retinal tissue using acoustic radiation force optical coherence elastography
Author Affiliations & Notes
  • Yueqiao (Rachel) Qu
    Beckman Laser Institute, University of California, Irvine, Irvine, California, United States
    Biomedical Engineering, University of California, Irvine, Irvine, California, United States
  • Youmin He
    Beckman Laser Institute, University of California, Irvine, Irvine, California, United States
    Biomedical Engineering, University of California, Irvine, Irvine, California, United States
  • Yi Zhang
    USC Roski Eye Institute & Institute for Biomedical Therapeutics, University of Southern California, Los Angeles, California, United States
  • Teng Ma
    USC Roski Eye Institute & Institute for Biomedical Therapeutics, University of Southern California, Los Angeles, California, United States
    NIH Ultrasonic Transducer Resource Center, Department of Biomedical Engineering, University of Southern California, Los Angeles, California, United States
  • Jiang Zhu
    Beckman Laser Institute, University of California, Irvine, Irvine, California, United States
  • Yusi Miao
    Beckman Laser Institute, University of California, Irvine, Irvine, California, United States
    Biomedical Engineering, University of California, Irvine, Irvine, California, United States
  • Cuixia Dai
    Beckman Laser Institute, University of California, Irvine, Irvine, California, United States
  • Ronald H Silverman
    Department of Ophthalmology, Columbia University Medical Center, New York, New York, United States
  • Mark S Humayun
    USC Roski Eye Institute & Institute for Biomedical Therapeutics, University of Southern California, Los Angeles, California, United States
  • Qifa Zhou
    USC Roski Eye Institute & Institute for Biomedical Therapeutics, University of Southern California, Los Angeles, California, United States
    NIH Ultrasonic Transducer Resource Center, Department of Biomedical Engineering, University of Southern California, Los Angeles, California, United States
  • Zhongping Chen
    Beckman Laser Institute, University of California, Irvine, Irvine, California, United States
    Biomedical Engineering, University of California, Irvine, Irvine, California, United States
  • Footnotes
    Commercial Relationships   Yueqiao (Rachel) Qu, None; Youmin He, None; Yi Zhang, None; Teng Ma, None; Jiang Zhu, None; Yusi Miao, None; Cuixia Dai, None; Ronald Silverman, None; Mark Humayun, None; Qifa Zhou, None; Zhongping Chen, OCT Medical Imaging (I), OCT Medical Imaging (C), OCT Medical Imaging (P)
  • Footnotes
    Support  R01HL-125084, R01HL-127271, R01EY-026091, R01EY-021529, and P41EB-015890), and the Air Force Office of Scientific Research (FA9550-14-1-0034)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3119. doi:
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    • Get Citation

      Yueqiao (Rachel) Qu, Youmin He, Yi Zhang, Teng Ma, Jiang Zhu, Yusi Miao, Cuixia Dai, Ronald H Silverman, Mark S Humayun, Qifa Zhou, Zhongping Chen; Quantified elasticity mapping of retinal tissue using acoustic radiation force optical coherence elastography. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3119.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Early diagnosis of age-related macular degeneration (AMD) remains a challenge due to limitations in functional imaging modalities in clinics today. It has been shown that the elasticity of the retina is altered during the onset of AMD due to drusen formation and neovascularization. This study shows the feasibility of using acoustic radiation force optical coherence elastography (ARF-OCE) to probe the elasticity of retinal layers to aid in the diagnosis of AMD.

Methods : We report on a minimally invasive ARF-OCE system that uses ARF excitation, and optically detects the displacement of the tissue spatially and in response to different frequencies. First, silicone phantoms are tested to validate the Voigt model relationship between the frequency response and the elastic modulus. Next, fresh ex vivo pig eyes were enucleated, and the displacement was recorded in 12 porcine retinas, in a 3 mm by 1 mm region on the temporal side, 2 mm from the optic disc. Segmentation is performed on the optical coherence tomography (OCT), such as in Figure 1A, and projected on to the displacement map, at 64 locations. Finally, the elastogram is generated using the frequency response, and a weighted average of the thickness and displacement for each layer across all areas.

Results : The OCE in Figure 1B shows the variation in displacement between the photoreceptor and ganglion sides of the retina. The elasticities of the layers are calculated and averaged across 64 scans (regions i. 1.33±0.37 kPa, ii. 2.73±0.82 kPa, iii. 7.7±2.26 kPa, iv. 25.9±7.36 kPa) and elastograms are generated for the corresponding location, shown in Figure 1C. The standard deviation is large for high strain regions due to small displacement. The OCT layers correlate to the H&E staining of the retina, where five distinct layers are matched in Figure 1D.

Conclusions : Our results validate the capability of ARF-OCE to quantify the elastic modulus of the retina. Only 2 samples were analyzed due to retinal detachment and other postmortem changes, so further confirmation in an in vivo model is required. This study is a potential stepping stone to clinical studies, where the elastogram can perhaps aid in the diagnosis of retinal diseases.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

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